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Transfer of human interferon γ-oleosin genes to safflower (Carthamus tinctorius L.)
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Athar Yaghtin   , Mokhtar Jalali Javaran * , Ghasem Karimzadeh |
| , Jalali.mokhtar@gmail.com |
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Abstract: (4694 Views) |
The use of plants as a source of medicine is traced back to long time ago. Modern biotechnology
proviedes the possibility of production of valuable protein such as pharmaceutical protein in
plant. In this study human Interferon gamma-oleosine genes under the control of Napin promoter
were transferred to Carthamus tinctorius L. (safflower) by Agrobacterium tumefaciens strain LBA4404.
Cotyledonal explants from safflower plant (Carthamus tinctorius L.) Padideh cultivar were used for
transformation. The transformed plants were screened on MS medium containing 0.09 mg L-1 NAA, and 1
mg L-1 TDZ containing 40 mg L-1 kanamycin. Presence of transgenes was confirmed using polymerase
chain reaction (PCR). Characterization of the transgenic plants is going on. |
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| Keywords: Agrobacterium, Carthamus tinctorius L., Human Interferon Gamma-Oleosin Genes, Molecular Farming, Transformation |
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Full-Text [PDF 33 kb]
(1399 Downloads)
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Type of Study: Research |
Subject:
Plant
Received: 2011/04/23 | Accepted: 2012/10/30 | Published: 2013/03/5
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