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:: Volume 6, Issue 1 (7-2017) ::
2017, 6(1): 65-75 Back to browse issues page
Proteomic Analysis of Spring Rapeseed Leaves under Salt Stress
Nima Dolatabadi , Mahmoud Toorchi , Mostafa Valizadeh , Ali Bandehagh
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Abstract:   (390 Views)
Excess salinity is one of the most important problems of agricultural production. Rapeseed is a superior oilseed due to the high quality of oil, large amounts of polyunsaturated fatty acids and oil yield. Rapeseed is classified as a semi-salt-tolerant plant although yields decrease more than in many other crops when salinity is higher than the threshold. Therefore breeding for increasing salt tolerance would be of interest. Changes of protein expression were investigated in a Safi7 tolerant genotype in order to identify the molecular mechanisms of salinity tolerance in rapeseed. NaCl concentrations of  0 (control), 150 and 300 mM caused a significant increase of Na+ content in leaves and a decrease of shoot dry weight, shoot height, leaf K+ content and the leaf K+/Na+ ratio. 110 repeatably-appearing protein spots were identified on two-dimensional electrophoresis (2-DE) gels and among them, 37 spots  showed significant expression changes based on changes in the induction factor (IF). Among them, 5 spots showed significant statistically changes at the 95% confidence level, with 1 spot being up-regulated and the other four spots down-regulated. Identification of the spots was performed by LC-MS/MS mass spectrometry analysis. The identified proteins play a key role in energy production and photosynthesis. Our results indicated that these proteins can also play a role in rapeseeds tolerance to salt stresses.
 
Keywords: Rapeseed, proteome, salt stress, two-dimensional electrophoresis, LC-MS/MS
Full-Text [PDF 389 kb]   (33 Downloads)    
Type of Study: Research | Subject: Divers
Received: 2017/03/14 | Accepted: 2017/07/10 | Published: 2017/08/10
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Dolatabadi N, Toorchi M, Valizadeh M, Bandehagh A. Proteomic Analysis of Spring Rapeseed Leaves under Salt Stress. Genetic Engineering and Biosafety Journal. 2017; 6 (1) :65-75
URL: http://gebsj.ir/article-1-191-en.html
Volume 6, Issue 1 (7-2017) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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