[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 6, Issue 1 (7-2017) ::
2017, 6(1): 77-84 Back to browse issues page
Identification and Cloning of a Cotton Reference Gene for Increasing Efficiency of Molecular Analysis
Masoud Tohidfar , Sara Dezhsetan
Dept of Plant Biotechnology, College of Science and Biotech, Shahid Beheshti University, Iran.
Abstract:   (475 Views)
The use of an appropriate reference gene for assessment of DNA and genome quality and analysis of transgenic crops is very important. In this research, we have evaluated the SadI gene as a potential reference gene in cotton. First, DNA was extracted, and then a PCR reaction was done using appropriative primers designed for Vector NTI. The PCR products were 623 and 544 bp fragments. The fragments were cloned in pTZ57R/T plasmid. Recombinant plasmids were identified by PCR colony hybridization with appropriative primers and M13 primers and then were sequenced. The sequencing results showed that these two fragments are related to two different copies of the SadI  gene that in one of copies a 79 bp deletion had occurred in the second interon. This research is the first report from sequencing of a reference gene showing the presence of two different copies of the SadI gene, and affirming that it is a suitable reference gene for different studies.
 
Keywords: Bioinformatics, Cloning, Cotton, Reference gene, sad1 gene
Full-Text [PDF 778 kb]   (51 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2017/05/7 | Accepted: 2017/07/10 | Published: 2017/07/10
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tohidfar M, Dezhsetan S. Identification and Cloning of a Cotton Reference Gene for Increasing Efficiency of Molecular Analysis. Genetic Engineering and Biosafety Journal. 2017; 6 (1) :77-84
URL: http://gebsj.ir/article-1-197-en.html
Volume 6, Issue 1 (7-2017) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
Persian site map - English site map - Created in 0.05 seconds with 792 queries by yektaweb 3531