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:: Volume 7, Issue 2 (12-2018) ::
2018, 7(2): 202-189 Back to browse issues page
Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.
Fatemeh Khakdan, Mojtaba Ranjbar *, Nader Chaparzadeh, Athar Sadat Javanmard, Houshang Alizadeh
Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol , ranjbarf@ausmt.ac.ir
Abstract:   (2122 Views)
Ocimum basilicum L. (2n=48) is a medicinal plant that belongs to Lamiaceae family. Eugenol is one of the most important components of basil essential oil which is utilized as an anti-bacterial and anti-fungal material in various industries. Phenylpropanoids such as methyl eugenol possess anti-oxidant properties against free radicals and act as protective compounds in plant responses to the biotic and abiotic stresses. Methyl eugenol O-methyl transferase (EOMTs), as a key enzyme of phenylpropanoids biosynthesis pathway, converts eugenol into methyl eugenol by methylation. In this study, we isolated EOMTs gene promoter from the basil plant and cloned in pTG19-T vector for sequencing. Then, this sequence w::as char::acterized and analyzed by bioinformatics softwares. We aimed to investigate the possible gene expression regulation of EOMTs gene under different environmental conditions and recognition different regulatory motifs of its promoter. Analysis of EOMTs gene promoter (1209 bp) indicated that this sequence included some important regulatory elements which could respond to high temperature and drought stress such as MYB, MYC, HSE, and WRKY. Transient expression of the GUS gene under ObEOMTs promoter control in Nicotiana tabacum leaves showed that this promoter including core elements was able to direct the basal expression of this gene. It is expected that this promoter will be capable of changing the expression levels of EOMTs gene in reaction to different environmental stresses because there are stress-responsive regulatory sites in ObEOMTs gene promoter.
Keywords: EOMTs promoter, cis-acting elements, drought stress, Ocimum basilicum L.
Full-Text [PDF 856 kb]   (528 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2019/01/2 | Accepted: 2019/03/17 | Published: 2019/04/20
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Khakdan F, Ranjbar M, Chaparzadeh N, Javanmard A S, Alizadeh H. Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.. Genetic Engineering and Biosafety Journal. 2018; 7 (2) :202-189
URL: http://gebsj.ir/article-1-274-en.html

Volume 7, Issue 2 (12-2018) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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