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:: Volume 7, Issue 2 (12-2018) ::
2018, 7(2): 217-230 Back to browse issues page
Haplotype Diversity For QTL Associated with Drought tolerance on Chromosome 2 of Rice
Parham Niksiar, Saeid Navvabpour *, Hosein Sabouri, Hasan Soltanlu, Masoud Rahimi
Gorgan University of Agricultural Sciences and Natural Resources. Iran , s.navabpour@gau.ac.ir
Abstract:   (1765 Views)
 In order to investigate the haplotype and allelic diversity of 22 rice (Oryza sativa L.) genotypes, a factorial experiment was conducted based on RCBD with three replications under normal (no stress) and drought stress (-5 bar) conditions at seedling stage. The 16 primer pairs of SSR markers related to drought tolerance were used for genotyping. The studied traits were included root diameter, root dry weight, root number, root length, stem dry weight, stem length, genotypic score and total biomass. Analysis of variance for studied traits showed that there was a significant genetic variation among genotypes. 16 used SSR loci produced 49 alleles. The number of alleles per locus generated by each primer pair varied from 2 to 6 alleles with an average of 3. The polymorphic information content (PIC) values ranged from 0.207 to 0.678 with an average of 0.462. RM8030 and RM3302 microsatellite markers were useful for discriminating between tolerant and susceptible genotypes and therefore may be useful for marker-assisted selection. 15 haplotypes were identified among these genotypes using Bala haplotype as reference for haplotype diversity. Genotypes of haplotype group 1 including Salari, CT6516-24-3-2, IR77298-14-1-2 and IR50 genotypes can be useful for breeding programs to drought tolerance.
Keywords: Haplotype diversity, PIC, Rice, SSR marker.
Full-Text [PDF 782 kb]   (464 Downloads)    
Type of Study: Research | Subject: Divers
Received: 2019/02/28 | Accepted: 2019/03/17 | Published: 2019/04/20
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Niksiar P, Navvabpour S, sabouri H, soltanlu H, rahimi M. Haplotype Diversity For QTL Associated with Drought tolerance on Chromosome 2 of Rice. Genetic Engineering and Biosafety Journal. 2018; 7 (2) :217-230
URL: http://gebsj.ir/article-1-289-en.html

Volume 7, Issue 2 (12-2018) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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