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:: Volume 8, Issue 1 (7-2019) ::
2019, 8(1): 91-100 Back to browse issues page
Changes in Protein Expression Patterns of Lime Leaves Caused by Witches' Broom Disease
Farzan Taheri *, Ghasem Hosseini
Department of Plant Breeding and Biotechnology Takestan Branch, Islamic Azad University, Takestan, Iran. , farzan_taheri@yahoo.com
Abstract:   (3123 Views)
"Witches' broom" Disease caused by the "Candidatus phytoplasma aurantifoliais one of the important citrus disease that causes significant economic losses in Iran, western Asia and North Africa. In this study the effect of disease stress on leaf proteome was investigated. Leaf samples were collected from plants and were analyzed using 2-DE coupled with MS. The power of 2DE for proteins separation makes it possible to study various qualitative and quantitative changes in protein expression under normal and stress condition. out of 801 leaf proteins from eight biological replicates of healthy and infected plants that were analyzed by melani6 software, 63 proteins showed a significant response to the disease. MS results indicated that 19 proteins were involved in oxidative stress defense photosynthesis, metabolism and the stress response. Identifying these proteins can reveal newer response pathways and the genes that control them. The results of this study can be used in mexican lime breeding programs.
Keywords: Witches’ Brooms, 2DE, Mass Spectrometry, Phytoplasma, Mexican lime
Full-Text [PDF 683 kb]   (1317 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2019/05/5 | Accepted: 2019/09/16 | Published: 2019/09/28
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Taheri F, Hosseini G. Changes in Protein Expression Patterns of Lime Leaves Caused by Witches' Broom Disease. Genetic Engineering and Biosafety Journal 2019; 8 (1) :91-100
URL: http://gebsj.ir/article-1-297-en.html


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Volume 8, Issue 1 (7-2019) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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