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Report on the occurrence and genetic diversity of melon yellow spot virus isolates in various provinces of Iran
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Mehrdad Salehzadeh *    , Alireza Afsharifar |
| Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran & Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran , mehrdadsalehzadeh@gmail.com |
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Abstract: (41 Views) |
| In a survey conducted in spring and summer 2024 across greenhouses in Isfahan, Shiraz, Bandar Abbas, Bushehr, and Yazd, severe symptoms such as yellow spots, mosaic patterns, leaf deformity, yellowing, fruit necrosis, and chlorotic ring spots were noted on cultivated C. sativus plants. To assess contamination with orthotospoviruses, total genomic RNA was extracted from five independent isolates from each area, along with an asymptomatic negative control, confirmed free of orthotospoviruses through molecular analysis. RT-PCR using degenerate primer pairs targeted orthotospoviruses, resulting in the amplification of a 420 bp fragment from infected samples. Further investigations revealed no mixed contamination with significant pathogenic viruses including Tobamoviruses, Potexviruses, Begomoviruses, and Potyviruses. Phylogenetic analysis based on nucleotide sequences indicated the presence of Melon Yellow Spot Virus. This marks the first recorded occurrence of Melon Yellow Spot Virus (MYSV) in Iran. |
Article number: 5 |
| Keywords: Iran, Orthotospovirus, Genetic diversity, Melon yellow spot virus. |
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Type of Study: Research |
Subject:
Microrganisms and Viruses
Received: 2024/12/23 | Accepted: 2025/03/1 | Published: 2025/08/4
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