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Optimization of callus induction in medicinal plant Cistanche tubulosa
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Fatemeh Akbari    , Fatemeh Zaker Tavallaie * , Masoumeh Salehi |
| Dept. of Plant Production and Genetics Engineering, Shirvan Agriculture Faculty, University of Bojnord, Bojnord, Iran. , f.zaker.t@um.ac.ir |
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Abstract: (49 Views) |
Abstract
Cistanche tubulosa, a holoparasitic perennial plant, is one of the most famous pasture and medicinal plants known as "desert ginseng". Many therapeutic properties have been reported for this plant. In this study, the callus formation of this plant was optimized. Initially, in order to determine the best disinfection treatment, plant parts were disinfected in five sodium hypochlorite treatments with concentrations of 1, 2, 3, 4 and 5% for 10 minutes and cultured in MS medium containing 3% sucrose and 10 g/L agar. By observing the browning of the explants, the effect of activated charcoal and ascorbic acid treatments on controlling the browning of the explants was investigated. In this study, different explants were used for callus formation. To investigate the effect of plant growth regulators on plant callus formation, explants were cultured on MS medium containing BAP at a concentration of 0.5 mg/L, kinetin at a concentration of 0.5 mg/L, and 2.4.D at twenty-three levels (0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, and 11 mg/L) in three replicates. This experiment was conducted in a completely randomized design. After 10 days, the probability of callus formation was examined for up to 2 months. The results showed that the lowest bacterial and fungal contamination in the explants was observed in the treatment of 2% sodium hypochlorite and 10 minutes of disinfection. The treatment of 0.5 g/L activated charcoal plus 0.5 g/L ascorbic acid was able to control browning well. The highest callus formation percentage was obtained in the treatment of 0.5 mg/L kinetin plus 0.5 mg/L BAP and 8.5 mg/L 2-4-D. The results of this study can be used in the future to perform indirect regeneration and also to produce active pharmaceutical ingredients in vitro using callus culture and cell suspension culture.
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Article number: 4 |
| Keywords: Cistanche tubolosa, Callus induction, 2, 4-D, Browning |
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Type of Study: Research |
Subject:
Plant
Received: 2025/02/2 | Accepted: 2025/04/29 | Published: 2025/09/4
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