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Showing 2 results for Azizpour

Samira Sanami, Maghsoud Pazhouhandeh, Rana Valizadeh Kamran, Kambiz Azizpour,
Volume 6, Issue 2 (3-2018)
Abstract

In this research, in order to study some physiological and molecular traits such as the expression level of Catalase (CAT1) and Ascorbate Peroxidase (APX1) genes in two, CH-Falat and Rio Grande-S, varieties of tomato plant (Solanum lycopersicum L.), an experiment was designed in randomized complete block design. Salinity with the concentration of 0, 25, 50, 75 and 100 mM NaCl was applied in hydroponic culture. Plants RNA was extracted and cDNA was synthesized, then Real–Time PCR was carried out. The results showed that the relative expression of CAT1 has been increased in Rio Grande-S at all levels of salinity and in Falat-CH variety only up to 50 mM NaCl. The relative expression of APX1 has been decreased in Rio Grande-S up to 50 mM of salinity level and has been increased in 75 and 100 mM salinity levels. The relative expression of APX1 in Falat-CH variety has been increased up to 50 mM salinity level. Some physiological traits in salinity treatments were also studied and the results showed that in both varieties, by salinity increase from 0 to 100 mM NaCl, the amount of Na, Prolin, the soluble sugers were increased and the amount of K, and K/Na were decreased. The membrane stability index was decreased in salinity treatments comparing to control. The results of the molecular and physiological analysis showed that Rio Grande S variety in comparison to CH-Falat is more resistant under high level of salinity.
Mahdi Motamedi, Kambiz Azizpour, Vahid Sarabi, Rana Valizade Kamran,
Volume 10, Issue 1 (8-2021)
Abstract

Despite the negative effects of salinity on plants, due to the limited area under cultivation, planting a variety of plants in saline lands is inevitable. Among them, okra is one of the plants that few studies have been done on its response to different levels of salt. Therefore, in order to study the effect of salinity levels on Okra (Abelmoschus esculentus Moench), an experiment was designed based on a randomized complete block design with five treatments including concentration of 0, 50, 100, 150 and 200 mM sodium chloride in three replications at the greenhouse of the faculty of agriculture, Shahid Madani University of Azarbaijan. The results showed that by increasing salinity levels, potassium to sodium ratio, chlorophyll a, relative water content, plant dry weight, number of fruits per plant, fruit weight per plant, number of seeds per plant, seeds weight per plant and 1000 grains weight have decreased but amount of sodium and proline have increased. During the experiment, the plants under 200 mM treatment have died shortly after salinization, but plants under other treatments survived until the end of the experiment and produced the yield. Maintaining high potassium content, low reduction in chlorophyll content and no noticeable change in relative water content were factors that led to continuance of plant growth, dry matter and fruit production at different salt concentrations. Although in terms of number of fruits per plant, there was no significant difference between salinity levels and the control, but a significant decrease in fruit weight per plant was recorded at high salt concentrations, especially under 150 mM, which can be an important factor affecting economic yield of okra in saline environments with EC higher than 10 ds/m.


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دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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