fa بررسی افزایش بیان ژنBnFUL در گیاهان تراریخته کلزا مهندسی ژنتیک گیاهی Plant پژوهشي Research <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلزا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یکی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مهم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روغنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سال</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اخیر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایران</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توسعه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زیادی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیدا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کرده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یکی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشکلات</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلزا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ریزش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قبل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برداشت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بسته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شرایط</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هوایی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تواند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تا</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> 50 </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">درصد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باعث</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کاهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محصول</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">متحمل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ریزش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تواند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">موجب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عملکرد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عوامل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنتیک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محیطی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">متعددی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">صفت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اثر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارند</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جمله</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عوامل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنتیک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">FUL</span></span></span></em></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تواند مانع</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شدن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">غلاف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ریزش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> ژن</span></span></strong> &nbsp;<strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">FUL</span></span></span></em></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلزا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رقم&nbsp; </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Quantum</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> جداسازی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به </span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سه رقم </span></span></strong>&nbsp;<strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انتقال</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> بررسی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مولکولی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نسل &nbsp;</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">T₂</span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آر، لکه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گذاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سادرن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Real Time-PCR</span></span></span></strong>&nbsp; <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">&nbsp;انجام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">علاوه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن داخلی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاه،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراژن</span></span></strong> <strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">BnFUL</span></span></span></em></strong>&nbsp; <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">&nbsp;نیز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">لکه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گذاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سادرن نشا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حداقل یک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نسخه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> . </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Real Time-PCR</span></span></span></strong> &nbsp;<strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن&nbsp; </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">BnFUL</span></span></span></em></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مقایسه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهان شاهد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یافته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">همچنین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سطح</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">د</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">لاین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نیز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اختلاف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان دادند</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بخشی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تفاوت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تواند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ناشی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تعداد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نسخه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اثرهای محل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ورود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنوم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باشد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong> Oilseed rape is one of the important oil plants that its cultivation has been expanded in Iran in recent years. Seed shattering before and during harvesting is a problem of cultivation of this plant can causes up to 50% yield decrease depending on the environmental conditions. Production of seed shatter tolerant plants can increase the yield. Different genetic and environmental factors can affect this trait. The <em>FUL </em>gene is one of the genetic factors in that its over expression could prevent silique opening and seed shattering. Rapeseed transgenic plants of three cultivars containing the <em>BnFUL </em>gene were produced and molecular studies were carried out on T2 generations transgenic plants using PCR, Southern blotting and real time PCR. PCR showed that the <em>BnFUL </em>transgene has been inserted in the transgenic plants in addition to the internal gene. The copy number of transgene in transgenic and control plants were analyzed by Southern blotting which confirmed presence of at least one intact copy of the transgene in transgenic plants. Real time PCR showed that <em>BnFUL </em>gene expression in transgenic plants in comparison with control plants has been increased. Differences between the expression of transgenic plants in different lines could be because of position effect and copy number. کلزا, گیاه تراریخته, بیان ژن BnFUL, تعداد نسخه, ریزش دانه 73 82 http://gebsj.ir/browse.php?a_code=A-10-255-1&slc_lang=fa&sid=1 Samira Kahak سمیرا کهک a_shakib@abrii.ac.ir 10031947532846001701 10031947532846001701 No Ali Mohammad Shakib علی محمد شکیب shakib@abrii.ac.ir 10031947532846001702 10031947532846001702 Yes Jalal Saba جلال صبا jsaba49@gmail.com 10031947532846001703 10031947532846001703 No Siamak Asadi سیامک اسدی 10031947532846001704 10031947532846001704 No Mana AhmadRaji مانا احمد راجی 10031947532846001705 10031947532846001705 No