Genetic Engineering and Biosafety Journal
مهندسی ژنتیک و ایمنی زیستی
Genetic Engineering and Biosafety Journal
Agriculture
http://gebsj.ir
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admin
2588-5073
2588-5081
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1111
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jalali
1391
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gregorian
2012
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online
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fulltext
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بررسی چند شکلی اگزون دو ژن BoLA-DRB3 در جمعیت گاومیش استان خوزستان با استفاده از روش PCR-RFLP
Investigation of bovine lymphocyte antigen (BoLA-DRB3) by PCR-based RFLP in buffalo population of Khuzestan province
مهندسی ژنتیک جانوری
Animal
پژوهشي
Research
<strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مجموعه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سازگاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بافت</span></span></strong> <strong><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">MHC</span></span></span></strong><strong><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کننده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنتی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پروتئین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سطح لوکوسیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دفاعی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بدن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شناسایی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پروتئین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خارجی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نقش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارند</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گاو</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بولا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">معروف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دسته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلاس</span></span></strong><strong><span dir="LTR"><span style="font-family:timesnewroman,bold;"><span style="font-size:12.0pt;">І</span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">، کلاس</span></span></strong> <strong><span dir="LTR"><span style="font-family:timesnewroman,bold;"><span style="font-size:12.0pt;">ІІ</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و کلاس</span></span></strong> <strong><span dir="LTR"><span style="font-family:timesnewroman,bold;"><span style="font-size:12.0pt;">ІІІ</span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> بر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بازوی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کوتاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کروموزوم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">23</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گاو</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ترتیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یافته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اند،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلاس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مجموعه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">متعدد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تواند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها آلل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شامل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> . </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هدف</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">چندشکلی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اگزون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">2</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنی </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">BoLA-DRB3</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جمعیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گاومیش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خوزستان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پی</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سی</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آر دو</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مرحله</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ای (</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Heminested-PCR</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">) برای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تکثیر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اگزون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خونگیری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">80</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رأس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گاومیش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شهرستان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">صورت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دو</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مرحله</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زنجیر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پلیمراز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت تکثیر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قطعه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">284</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جفت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بازی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اگزون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">2</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">MHC</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آغازگرهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اختصاصی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن انجام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قطعه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تکثیر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وسیله</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">III</span></span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Hae</span></span></span></em><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> و </span></span></strong>RsaІ <strong><strong><span dir="LTR"><span style="font-family:timesnewroman,bold;"><span style="font-size:12.0pt;"> </span></span></span></strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تیمار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> نتایج</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حاصل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">III</span></span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Hae</span></span></span></em></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">، نه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">الگوی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جایگاه ژنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">الگوهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی </span></span></strong> <strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">a</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">b </span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ترتیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فراوانی 34/37</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و 23/75 درصد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیشترین فراوانی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آللی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اختصاص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دادند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">17</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ترکیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنتیکی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شناسایی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> آنزیم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Rsa</span></span></span></em></strong><strong><span dir="LTR"><span style="font-family:timesnewroman,bold;"><span style="font-size:12.0pt;">І</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">، تعداد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">الگوی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">الگوهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برشی </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">g</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> و</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">b</span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ترتیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فراوانی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">30</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">20</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">درصد بیشترین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فراوانی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آللی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اختصاص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دادند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نیز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">17</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ترکیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنتیک شناسایی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> . </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مربع</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جمعیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حالت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تعادل هاردی</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">-</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وینبرگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> . </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حاضر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سطح</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بسیار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بالایی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پلی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورفیسم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جمعیت گاومیش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خوزستان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشاهده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><br>
MHC locus codes antigens and leukocyte surface proteins which have roles on immune reactions and identification of foreign proteins. In cattle this locus is known as Bulla and is composed of three classes of genes: CLASS І, CLASS ІІ and CLASS ІІІ and is located on short arm chromosome 23. Each of these classes of genes has complex and various genes and each gene may have dozens of alleles. The objective of this study was to study the variation of exon 2 of locus BoLA-DRB3 in buffalo population in Khuzestan province. In this study, the Heminested-PCR method was used to amplifing this exon. In order to determain the level of polymorphism, blood sample were collected from 80 buffalos in Shadegan, Ahwaz, Dashte azadegan, Dezfoul and Shoshtar cities. DNA extraction and exon 2 of the MHC gene was amplified by specific set of primers for this gene to produce a 284 bp fragment. The amplified fragments were digested with <em>Hae</em>III and <em>Rsa</em>І restriction endonuclease. Digested products were separated and were stained by vertical electrophoresis on 8% Polyacrylamide gel. After digestion with <em>Hae</em>III and <em>Rsa</em>І nine and ten alleles (restriction digestion pattern) were obtained at this locus respectively. Alleles a and b with 34.37 and 23.75 percent were the most frequent alleles when digested with <em>Hae</em>III and alleles a and b with 20 and 30 percent frequency were the frequent alleles when digested with <em>Rsa</em>І. We identified 17 genotypes using each of the restriction enzymes in this population.
پلی مورفیسم, ژن MHC ,PBR , گاومیش
Polymorphism, MHC, Buffalo, PBR
121
128
http://gebsj.ir/browse.php?a_code=A-10-259-1&slc_lang=fa&sid=1
Somayeh
Rahimnahal
سمیه
رحیم نهال
S.Rahimnahal@yahoo.com
10031947532846001715
10031947532846001715
Yes
Jamal
Fayazi
جمال
فیاضی
j_fayazi@yahoo.com
10031947532846001716
10031947532846001716
No
Khalil
Mirzadeh
خلیل
میرزاده
mirzadeh2005@gmail.com
10031947532846001717
10031947532846001717
No
Mohammad Taghi
Beigi Nassiri
محمد تقی
بیگی نصیری
mt_nassiri@hotmail.com
10031947532846001718
10031947532846001718
No
Hedayat Allah
Roshanfekr
هدایت الله
روشنفکر
roshanfekr_hd@cua.ac.ir
10031947532846001719
10031947532846001719
No