fa مطالعه بیان عوامل رونویسی WRKY و AP2Domain در آفتابگردان روغنی تحت تنش شوری تخصصی متفرقه Divers پژوهشي Research <div><strong><span style="color:black;"><span style="font-family:B Zar;">آفتابگردان (</span></span></strong><strong><em><span dir="LTR"><span style="color:black;">Helianthus annuus</span></span></em></strong><strong><span dir="LTR"><span style="color:black;"> L.</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">) از</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مهم‌ترین</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">منابع</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">روغن</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">گیاهی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">در</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">جهان</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">و</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">ایران</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">است. این</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">گیاه</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">جزء</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">گیاهان</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">نیمه</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مقاوم</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">به شوری دسته</span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:B Zar;">&lrm;</span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">بندی می‌شود،</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">با</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">این‌حال،</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">عملکرد</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">آن</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">به</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">شکل</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">منفی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">تحت</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">تاثیر</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">شوری</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">قرار</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">می‌گیرد. عوامل‌ رونویسی مولکول‌هایی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">هستند</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">که</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">نقش</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مهمی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">در</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">درک</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">و</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">انتقال</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">پیام</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">تنش</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">و</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">نیز</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">بسیاری</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">از</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">فرآیندهای</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">فیزیولوژیکی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">دارند. در</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">این</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مطالعه با به کار‌گیری فناوری واکنش زنجیره&shy;ای پلی&shy;مراز در زمان واقعی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">تغییرات بیان دو</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">عامل</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">رونویسی</span></span></strong><strong><span dir="LTR"><span style="color:black;"> WRKY </span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">&nbsp;و</span></span></strong><strong><span dir="LTR"><span style="color:black;"> AP2Domain </span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">&nbsp;در شرایط مختلف تنش شوری (2، 5، 8، 11 و 14 دسی زیمنس بر متر) در دو لاین مختلف آفتابگردان روغنی (</span></span></strong><strong><span dir="LTR"><span style="color:black;">AS5305</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> و </span></span></strong><strong><span dir="LTR"><span style="color:black;">9CSA3</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">) بررسی شد.</span></span></strong><strong><span style="layout-grid-mode:line;"><span style="color:black;"><span style="font-family:B Zar;"> نمونه‌برداری از برگ‌های گیاهان در مرحله 8 برگی در پنج زمان 0، 6، 12، 24 و 48</span></span></span></strong> <strong><span style="layout-grid-mode:line;"><span style="color:black;"><span style="font-family:B Zar;">ساعت پس از اعمال تنش شوری انجام شد. نتایج تجزیه&shy;های آماری نشان داد</span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> الگوی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">بیان</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">ژن‌های</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">کدکننده</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">عوامل</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">رونویسی</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مورد</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">مطالعه</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">در</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">لاین‌های مورد </span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">بررسی</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> متفاوت است به‌طوری که میزان بیان نسبی هر دو عامل رونویسی (</span></span></strong><strong><span dir="LTR"><span style="color:black;">WRKY</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> و </span></span></strong><strong><span dir="LTR"><span style="color:black;">AP2Domain</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">)</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">در لاین </span></span></strong><strong><span dir="LTR"><span style="color:black;">AS5305</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> (لاین متحمل) نسبت به لاین </span></span></strong><strong><span dir="LTR"><span style="color:black;">9CSA3</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> (لاین حساس) در شوری&shy;های شدید (به ترتیب در 11 و 8 دسی زیمنس بر متر) 24 ساعت بعد از اعمال تنش به‌طور فاحش افزایش یافته است.</span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;">نتایج نشان‌دهنده‌ نقش مثبت عوامل رونویسی</span></span></strong><strong><span dir="LTR"><span style="color:black;">AP2 Domain </span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;">&nbsp;و </span></span></strong><strong><span dir="LTR"><span style="color:black;">WRKY</span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"> در مکانیسم مقاومت آفتابگردان به تنش شوری می‌باشد.</span></span></strong><strong><span dir="LTR"><span style="color:black;"></span></span></strong></div> <div></div> Sunflower is one of the most important sources of plant oil in the world and in Iran. This plant is classified as semi-resistant to salt stress; however, its performance is negatively affected by salinity. Transcription factors are molecules that play an important role in the understanding and transmission of stress messages as well as many physiological processes. In this study, using real time polymerase chain reaction technology, transcript variations of two transcription factors WRKY and AP2Domain were examined in two oily sunflower lines; AS5305 (salt tolerant line) and 9CSA3 (salt susceptible line) at different salinity levels (2, 5, 8, 11, 14 ds/m). Sampling from leaves of plants was performed at 8-leaf stage in 0, 6, 12, 24, 48 hours after applying stress. The results of statistical analysis showed that the gene expression pattern of studied transcription factors was different in sunflower lines. So that, the rate of expression of both studied transcription factors (WRKY and AP2Domain) was significantly more in tolerant line (AS5305) than susceptible one in sever salt stress level (11 and 8 ds/m, respectively) 24 hours after applying stress. The results indicate the positive role of AP2Domain and WRKY transcription factors in the resistance mechanism of sunflower to salt stress.<strong><span dir="RTL"></span></strong> آفتابگردان, NaCl, بیان ژن, مقاومت به شوری, واکنش زنجیره ای پلی مراز در زمان واقعی. NaCl, gene expression, real time PCR, salt resistance, sunflower. 178 188 http://gebsj.ir/browse.php?a_code=A-10-232-2&slc_lang=fa&sid=1 Faezeh Hoseinpour فائزه حسین پور faeze_hoseinpor@yahoo.com 10031947532846003100 10031947532846003100 No Urmia University گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه Reza Darvishzadeh رضا درویش زاده r.darvishzadeh@urmia.ac.ir 10031947532846003101 10031947532846003101 Yes Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه، ایران Babak Abdollahi بابک عبدالهی b.abdollahi@urmia.ac.ir 10031947532846003102 10031947532846003102 No Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه