Genetic Engineering and Biosafety Journal
مهندسی ژنتیک و ایمنی زیستی
gebsj
Agriculture
http://gebsj.ir
1
admin
2588-5073
2588-5081
1
10.61882/gebsj
11
1111
11
fa
jalali
1400
5
1
gregorian
2021
8
1
10
1
online
1
fulltext
fa
اثربخشی سویههای باسیلوس تولید کننده اکسین بر صفات رویشی و بیان نسبی ژن ARF15در دو ژنوتیپ نخود در شرایط تنش شوری
Efficacy of Auxin-producing Bacillus Strains on Vegetative Traits and Relative Expression of ARF15 Gene in Two Genotypes of Chickpea Under Salinity Stress
مهندسی ژنتیک گیاهی
Plant
پژوهشي
Research
<div style="text-align: justify;"><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">شوری خاک به</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Calibri,sans-serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">عنوان یکی از مهم</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">ترین مشکلات کشاورزی، علاوه بر اثر منفی بر تولید محصولات، منجر به عدم</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">­تعادل هورمونی</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">گیاه و کاهش رشد میشود. ریزوباکتریهای محرک رشد گیاه (</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">PGPR</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">)</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">شامل طیفی از سویههای باکتری از گروههای مختلف است که در ریشه گیاهان و ریزوسفر زندگی میکنند و با ایجاد تغییرات پیچیده در رشد و نمو گیاهان، میتوانند مقاومت به تنش را افزایش دهند.</span></span></span></strong> <strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">PGPR</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> با تغییر در </span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">سوخت</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">و</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">ساز</span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> گیاه، سیگنالینگ و هومئوستازی هورمون همراه است. در این پژوهش اثر دو سویه باسیلوس</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">تولید کننده اکسین بر صفات رویشی و</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">بیان نسبی ژن</span></span></span></strong><strong><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">ARF15</span></span></span></span></em></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> در دو ژنوتیپ نخود (</span></span></span></strong><strong><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">Cicer arietinum</span></span></span></span></em></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">) (</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">MCC77</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> و</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">MCC68</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">) تحت تنش شوری 50 و 100 میلیمولار کلریدسدیم در شرایط گلخانه مورد ارزیابی قرار گرفت. تعداد شاخه ­های فرعی</span></span></span></strong> <em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">ژنوتیپ</span></span></span></span></strong></em> <strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">MCC68</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> در تلقیح </span></span></span></strong><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">سویه ­ها </span></span></span></span></strong></em><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">با غلظت 5/1،0 در شرایط 50 میلی</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">مولار کلرید</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">سدیم افزایش معنی</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">دار نشان داد. در </span></span></span></strong><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">تلقیح </span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">Bacillus cereus </span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">، (</span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">FZB42</span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">) </span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">Bacillus amyloliquefaciens</span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> با غلظت 5/0، 1 بر وزن تر</span></span></span></span></strong></em> <em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">ریشه </span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">MCC77</span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> افزایش معنی­ دار مشاهده شد</span></span></span></span></strong></em><em><strong><span style="background:white;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">.</span></span></span></span></strong></em> <em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">FZB42</span></span></span></span></strong></em> <em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">در غلظت 1 موجب افزایش معنی</span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">دار 20/28 درصد ارتفاع گیاهان </span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">MCC77</span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> در سطح 100 میلی</span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">مولار</span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> کلرید</span></span></span></span></strong></em><em><strong><span dir="LTR" style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong></em><em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">سدیم شد.</span></span></span></span></strong></em> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">همچنین، بیان نسبی ژن </span></span></span></strong><strong><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">ARF15</span></span></span></span></em></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> ژنوتیپ </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"> MCC68</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">در تیمار تلقیح شده با </span></span></span></strong><em><strong><span dir="LTR" style="background:white;"><span style="color:black;"><span style="font-family:Times New Romans,serif;"><span style="font-size:9.0pt;">Bacillus sp. </span></span></span></span></strong></em> <em><strong><span style="background:white;font-style:normal;"><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">در غلظت 5/0 و</span></span></span></span></strong></em><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> 50 میلیمولار کلریدسدیم، افزایش 9 برابری نشان داد.</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">هر دو سویه </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">FZB42</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> و </span></span></span></strong><strong><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">B. cereus</span></span></span></span></em></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> بر صفات رویشی ژنوتیپ­های نخود </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">MCC68</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> و </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">MCC77</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;"> تأثیر گذاشتند که بیانگر نقش </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">PGPR</span></span></span></span></strong> <strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">در افزایش تأمین متابولیت</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">های آنزیمی و غیرآنزیمی و بیان ژن­های گیاه در پاسخ به تنش</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:B Zar;"><span style="font-size:9.0pt;">های غیرزنده است.</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;"></span></span></span></span></strong></div>
<div style="text-align: justify;">Soil salinity is one of the most crucial agricultural problems that in addition to having negative effects on crop production, causes hormonal imbalance and growth reduction in the plant. Plant growth-promoting rhizobacteria (PGPR) are a range of bacterial strains from different groups that live in plant roots and rhizospheres and can cause the plants to withstand stress by causing complex alterations in their growth and development. PGPR alters plant metabolism, signaling, and hormone homeostasis. The present study aimed to evaluate the effect of two auxin-producing <em>Bacillus</em> strains on vegetative traits and the relative expression of the <em>ARF15</em> gene of two genotypes of chickpea (<em>Cicer arietinum</em>) (MCC77 and MCC68) under salinity stress of 50 and 100 mM sodium chloride in greenhouse conditions. The number of sub-branches of MCC68 has been significantly increased in the inoculation of strains at the concentrations of 0.5 and 1 in the presence of 50 mM sodium chloride. Moreover, the inoculation of <em>Bacillus amyloliquefaciens</em> (FZB42) and <em>Bacillus. cereus</em> at a concentration of 0.5 and 1 led to a significant increase in the fresh weight of MCC77 root<span dir="RTL">.</span> In addition, FZB42 at the concentration of 1 caused a significant increase (28.20%) in the height of MCC77 plants in the presence of 100 mM sodium chloride. In the inoculation of <em>Bacillus</em> sp. at the concentration of 0.5 and the presence of 50 mM sodium chloride, the relative expression of the ARF15 gene of MCC68 genotype showed a 9-fold increase. Both FZB42 and <em>B. cereus</em> strains affected the vegetative traits of MCC68 and MCC77 genotypes, which indicated the role of PGPR in increasing the supply of enzymatic and non-enzymatic metabolites, and the expression of plant genes in response to abiotic stresses.</div>
باسیلوس, شوری, ژن ARF15, نخود
ARF15 gene, Bacillus, Chickpea, Salinity
93
107
http://gebsj.ir/browse.php?a_code=A-10-425-1&slc_lang=fa&sid=1
azam
Rahimi chegeni
اعظم
رحیمی چگنی
rahimiazam20@yahoo.com
10031947532846004318
10031947532846004318
No
Science and Research Branch, Islamic Azad University, Tehran, Iran
گروه بیوتکنولوژی و بهنژادی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Foad
Fatehi
فواد
فاتحی
fatehi.foad@gmail.com
10031947532846004319
10031947532846004319
Yes
Department of Agriculture, Payame Noor University (PNU), Tehran, Iran
گروه کشاورزی، دانشگاه پیام نور، تهران، ایران
Asa
Ebrahimi
آسا
ابراهیمی
dr.asaebrahimi@gmail.com
10031947532846004320
10031947532846004320
No
Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
گروه بیوتکنولوژی و بهنژادی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی تهران، ایران
Mahmood
Maleki
محمود
ملکی
maleki.li@gmail.com
10031947532846004321
10031947532846004321
No
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
گروه بیوتکنولوژی و بهنژادی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران