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:: Volume 2, Issue 2 (10-2013) ::
2013, 2(2): 127-134 Back to browse issues page
Identification of PEPCK-C polymorphism using PCR- RFLP method and its association with economic traits in native fowl of Khorasan Razavi province
Feresht Ghadamgahi , Saeed Zerehdaran , Mokhtar Ali Abbasi , Mojtaba Ahani Azari * , Mona Salehi Nasab
Gorgan University of Agricultural Sciences and Natural Resources , mojtaba-9@yahoo.com
Abstract:   (5604 Views)

this study aimed to determine the PEPCK-C gene polymorphism in a population of native fowl in Khorasan Razavi. This gene encodes an enzyme with the same name in most organisms. This enzyme plays a key role in the gluconeogenesis. There are two types of this gene namely, cytosolic (PEPCK-C) and mitochondrial (PEPCK-M) types. In this study, blood samples were randomly collected from 100 native fowl of Khorasan Razavi. DNA was extracted using modified salting out method and then a fragment with the length of 1000 bp using a pair of primers was amplified. This fragment covers the promoter and exon 3 of PEPCK-C gene. For genotyping, the PCR-RFLP method using the BstEII digesting enzyme was performed. The GLM procedure of SAS 9.1 was used to study the association of the PEPCK-C gene polymorphism with economic traits. Three genotype patterns including AA, AB and BB were observed with frequencies of 0.46, 0.35 and 0.19, respectively. The effects of genotypes were significant for birth weight (P<0.01), weight at 12 weeks of age (P<0.0001) and average egg weight between 28 to 32 weeks of age (P<0.002). Other studied traits were not significantly influenced by genotypes of this gene. Based on the results of this study the BB genotype had the greatest impact on birth weight, weight at 12 weeks of age and average egg weight. Therefore, the selection based on the polymorphism of this gene could be useful for improving body weight and egg weight of Khorasan Razavi native fowl.
Keywords: PEPCK-C gene, Economic Traits, Polymorphism, Khorasan Razavi, Native fowl
Full-Text [PDF 354 kb]   (1702 Downloads)    
Type of Study: Research | Subject: Animal
Received: 2013/09/13 | Accepted: 2014/01/29 | Published: 2017/02/18
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Ghadamgahi F, Zerehdaran S, Abbasi M A, Ahani Azari M, Salehi Nasab M. Identification of PEPCK-C polymorphism using PCR- RFLP method and its association with economic traits in native fowl of Khorasan Razavi province. Genetic Engineering and Biosafety Journal 2013; 2 (2) :127-134
URL: http://gebsj.ir/article-1-181-en.html
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Volume 2, Issue 2 (10-2013) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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