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:: Volume 8, Issue 1 (7-2019) ::
2019, 8(1): 111-120 Back to browse issues page
Increased digestibility of milk beta-lactoglobulin by treatment with a plant recombinant glutathione/ thioredoxin system
Tahere Shahriari farfani * , Azar Shahpiri
Biotechnology Dept., Agriculture Faculty, Isfahan University of Technology, Iran , tahereshahriari2015@gmail.com
Abstract:   (3639 Views)

Β-lactoglobulin (BLG) is one of the main allergens in cow’s milk that causes it to be digested poorly. BLG structure has two intramolecular disulfide bonds and one free cysteine in cys121 that disulfide bonds are responsible for its low digestibility. It seems that the digestibility of BLG will be increased via the reduction of disulfide bonds. In the present work, the aim was to study the effect of plant glutathione/thioredoxin systems on the digestibility of milk BLG. Thioredoxins (Trxs) are small and abundant disulfide reductase in all organisms. they are reduced with Trx reductase or glutathione reductase or glutathione. Previously the genes encoding plant Trx, OsTrx20 was cloned and heterologously expressed in E.coli. In this work this protein was produced and purified in considerable amounts. The interaction of Trx with glutathione (GSH) was confirmed by insulin assay. The result of this assay showed that GSH was able to reduce OsTrx20. Therefore, in this work the effect of GSH/OsTrx20 on the digestibility of BLG was studied. Finally, BLG was pre-treated by GSH/OsTrx20 at 4, 25 and 37°C and then digested with trypsin. The digestibility was studied by using SDS-PAGE and HPLC and allergenicity was studied by ELISA. The results revealed that the GSH/Trx system significantly affects on the BLG digestibility and allergenicity.
 

Keywords: glutathione, beta lactoglobulin, digestibility, allergenicity, reverse phase -liquid chromatography
Full-Text [PDF 511 kb]   (1318 Downloads)    
Type of Study: Research | Subject: Divers
Received: 2019/05/19 | Accepted: 2019/10/3 | Published: 2019/10/8
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Shahriari farfani T, Shahpiri A. Increased digestibility of milk beta-lactoglobulin by treatment with a plant recombinant glutathione/ thioredoxin system. Genetic Engineering and Biosafety Journal 2019; 8 (1) :111-120
URL: http://gebsj.ir/article-1-303-en.html


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Volume 8, Issue 1 (7-2019) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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