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Evaluation of antioxidant activity, phenolic contents and pigment level of rolC and trolC transgenic tobacco plants
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Gita Amini   , Hanieh Mohajjel shoja * , Rohollah Motafakker azad , Elham Mohajel kazemi |
| Department of Plant Biology, Faculty of Natural Science, University of Tabriz, IRAN , mohajelh@yahoo.com |
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Abstract: (4191 Views) |
| Agrobacterium is one of the gram negative genera of soil bacteria that causes many diseases in plants through the use of horizontal gene transfer. The rol genes of A. rhizogenese, rolA, rolB and rolC, are the major causes of ‘hairy root’ symptoms in infected plants. It has been demonstrated that rol genes could have a positive effect on secondary metabolism in plants. The homologue of rol genes has also been found in some plants such as the genus Nicotiana and a member of Scrophulariaceae, Linaria vulgaris. Here we examined the effect of A. rhizogeneses rolC gene and its homologue in tobacco, trolC gene, on pigment content, production of some secondary metabolites and antioxidant activity in ‘in vitro’ cultured 4-week old seedlings. In Nicotiana plants, the transgenes were located under the control of dexamethasone inducible prompter. Methanol extracts of aerial parts of seedlings were prepared and DPPH radical scavenging method was used to evaluate the antioxidant activity. Experiments were performed in completely randomized design with 3 replications. Results showed that in induced transgenic lines, pigments content (chlorophyll a, chlorophyll b, carotenoid and anthocyanin) were decreased and total phenol contents were significantly increased compared to non-induced plants (control) and DPPH radical scavenging activity exhibited a remarkable increase in induced plants. However, the level of flavonoids didn’t show a significant change. These results indicate that plant homologue of bacterial rolC gene, trolC, functions as rolC and can be used as an alternative to enhance the level of secondary metabolite in plants. |
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| Keywords: rol genes, Nicotiana tabacum, antioxidant activity, phenolic content |
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Full-Text [PDF 833 kb]
(1298 Downloads)
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Type of Study: Research |
Subject:
Plant
Received: 2019/09/16 | Accepted: 2019/12/6 | Published: 2020/02/3
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