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Physiological response and expression of genes involved in drought tolerance in tolerant and susceptible bread wheat cultivars
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Foad Fatehi *   , Hamid Mohammadi |
| Department of Agriculture, Payame Noor University (PNU), Tehran, Iran , fatehi.foad@gmail.com |
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Abstract: (5218 Views) |
| Water stress is one of the most important environmental factors that regulate plant growth and development, and limit production. Plants can respond and adapt to water stress by altering their cellular metabolism and invoking various defense mechanism. Manipulating the levels of antioxidative defense system may affect yield. Therefore, the aim of this study was to find out the relationship between gene expression of antioxidant enzymes and proline as an osmotic regulator in wheat cultivars under drought stress. For this purpose, a factorial experiment based on randomized complete block design was carried out with three replications. Treatments were two Iranian wheat cultivars (Pishtaz and Niknejad) and three levels of drought stress. Drought stress resulted in a reduction of Chl a, Chl b, total Chl and yield in both cultivars. This reduction was lower in tolerant cultivar Niknejad. Drought stress led to an enhancement in proline content, the activity of CAT (Catalase), APX (Ascorbate Peroxidase) and SOD (Superoxide Dismutase) enzymes. The tolerance of Niknejad cultivar under drought stress is associated with high chlorophyll (Chlorophyll), proline contents which is closely related to its enzymatic antioxidants activity (including CAT, APX and SOD). Gene expression analysis also showed that expression levels of CAT, APX, SOD and P5C5 genes increased under drought stress. Niknejad cultivar had a better antioxidant capacity and had a higher induction of stress-related genes under drought stress. Therefore, this cultivar performs better than the Pishtaz under stress conditions. Among the genes studied, it seems that SOD and P5C5 genes played important roles in response to drought stress conditions. |
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| Keywords: Yield, Antioxidants, Proline, Gene Expression, Drought stress, Wheat. |
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Full-Text [PDF 1050 kb]
(1430 Downloads)
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Type of Study: Research |
Subject:
Plant
Received: 2019/10/25 | Accepted: 2019/12/12 | Published: 2019/12/20
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