The residual of pesticides in the agricultural production is endangering human health and environmental balance. Concerns about pesticide residues and environmental impacts due to repeated use of pesticides has led to investigations of the residue and fate of these agents. The adverse effects of pesticides' residue on humans and the environment have increased interest in genetic engineering technology and cultivation of genetically modified crops for pest management strategies. This study was carried out to investigate the residue of permethrin (EC 25%) in a Vendor cultivar of tomato in greenhouse.  Tomato plants were sprayed at doses of 0.5- 1 g/lit. Samples preparation was performed by the QuEChERS method. Further purification was achieved using a silica solid-phase extraction (SPE) cartridge and pesticide residues were analyzed using GC-MS. This study revealed that residue decreased in descending order. Results showed that the Permethrin (0.5, 1 g/lit) levels were detected below than maximum residue level (MRL) recommended by codex (0.5 mg/kg) at 5 and 7 days after application. Permethrin levels (1 g/lit treatment) were detected below MRL 10 days after application.