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Antiproliferative potential of Lactobacillus casei supernatant against MCF-7 breast cancer and HT-29 colorectal cancer cell lines
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Zahra Haji Mehdi Nouri    , Kumarss Amini , Farzaneh Tafvizi * , Nooshin Khandandezfoli , Babak Kheirkhah |
| Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran , farzanehtafvizi54@gmail.com |
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Abstract: (96 Views) |
| TThis study aimed to investigate the induction of cytotoxicity in colon (HT-29) and breast (MCF-7) cancer cells after treatment with the supernatant of Lactobacillus casei bacteria, as well as changes in the expression of genes involved in apoptosis in vitro. After extracting the bacterial supernatant, the MTT test was performed to check the viability of the examined cancer cells treated with different concentrations of the supernatant, and the expression of genes involved in apoptosis was measured using the Real-Time PCR technique in the treated cells. Concentrations of 3.125 (P<0.01), 6.25, 12.5, 25, 50 and 100 (P<0.001) μg/mL of bacterial supernatant caused a significant decrease in MCF-7 cancer cell viability. Also, a significant decrease in the survival of HT-29 cancer cells was observed under the treatment with concentrations of 12.5, 25, 50 and 100 μg/mL. It was also shown that the IC50 concentration of the supernatant for two cancer cell lines, HT-29 and MCF-7, is associated with increased expression of pro-apoptotic genes Caspase 3 (CASP3), Caspase 9 (CASP9), BCL2 associated X regulator (BAX) and decreased expression of BCL2 apoptosis regulator (BCL2), which ultimately induces apoptosis and programmed death cell in both types of cells. Negligible cytotoxicity was observed in normal HFF cells compared to cancer cells. The results of the study showed that the supernatant of Lactobacillus casei has an effective ability and significant potential in dealing with breast and colorectal cancer cells. |
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| Keywords: Apoptosis, Breast cancer, Colorectal cancer, Lactobacillus casei, Supernatant |
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Type of Study: Research |
Subject:
Divers
Received: 2023/07/7 | Accepted: 2023/09/27 | Published: 2024/03/14
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