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Transformation of human IFN-α (HU-IFN-α) gene to Urmia cultivar of tomato using Agrobacterium tumefaciens
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Vahid Mehrizadeh    , Ebrahim Dorani * |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran , dorani@tabrizu.ac.ir |
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Abstract: (63 Views) |
Interferons, especially human interferon Alpha, have high therapeutic and economic value and there is a strong interest in producing them from stable, safe and inexpensive sources such as plants. The aim of this study was investigated the possibility of transformation of tomato with human interferon Alpha. For this purpose, some of the effective factors on the transfer of the human IFN-α gene to tomato, such as the type of explant, Agrobacterium concentration (OD600nm = 0.2, 0.4, 0.6, 0.8 and 1), inoculation time (5, 10, 15, 20 and 25 minutes), co-cultivation time (1, 2, 3 and 4 days) and different concentration of the hygromycin (0, 7.5, 10, 15 and 20 mg/l) were investigated. The results showed that the cotyledon explants were the most suitable explant and 15 mg/L of hygromycin was the effective concentration of the antibiotic in transformation of tomato. Also, the highest percentage of transformation of tomato explants was observed in the OD600nm= 0.6 of Agrobacterium, 20 minutes of inoculation and 3 days of co-cultivation. Finally, the presence of human IFN-α gene in the probable transgenic plants was confirmed by the PCR technique and specific primers.
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Article number: 7 |
| Keywords: Agrobacterium, Transformation, Interferon Alpha, Recombinant Protein, Tomato |
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Type of Study: Research |
Subject:
Plant
Received: 2025/03/11 | Accepted: 2025/08/27 | Published: 2025/10/28
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