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:: Volume 4, Issue 1 (4-2015) ::
2015, 4(1): 25-39 Back to browse issues page
The Effect of Nano Cobalt and Nano Chitosan on Artemisinin production and expression of SQS and DBR2 genes in Artemisia annua
Bita Ghassemi , Ramin Hosseini * , Fatemeh Dehghan nayeri
International University, Gazvin, Iran
Abstract:   (5457 Views)

artemisia annua is particularly important for the production of artemisinin, a bioproduct which can be used to combat the causal agent of malaria, treat some kind of cancers and  in other activities. The low artemisinin content in the plant has caused  this compound to be among the more expensive medicines. Several attempts have been made to increase artemisinin production, for example by using different elicitors, but none of the approaches has been cost effective. In this study, the expression levels of two important genes in the artemisinin biosynthetic pathway, SQS and DBR2 and artemisinin content were investigated in Artemisia cell suspension cultures. SQS and DBR2 genes have essential roles in the regulation of artemisinin pathway. For this purpose, nano-cobalt particles in concentrations of 0.25, 2.5 and 5 mg/L were used for cell culture treatment and samples were collected after 8, 24, 48 and 72 h. The highest artemisinin content was observed 24 h after 5 mg/L nanocobalt treatment. In this case, artemisinin production was 2.25 times (113.35 mg/g d.wt) higher than that of the control. Our results showed a negative and significant correlation between SQS and DBR2 gene expression and artemisinin content at different levels of nano cobalt treatments. Results also showed an increase in nano cobalt concentration after 72 hour and an increase in nano chitosan after 4h hour caused a significant decrease in the expression of SQS and DBR2 genes. In conclusion, it appears that the content of artemisinin was increased by high concentrations of the nano cobalt particles because of a decrease in the expression of SQS and DBR2 genes

Keywords: Artemisia annua, Artemisinin, Nano Cobalt, Nano Chitosan, HPLC, qRT-PCR
Full-Text [PDF 540 kb]   (3768 Downloads)    
Type of Study: Research | Subject: Animal
Received: 2015/02/7 | Accepted: 2015/08/3 | Published: 2017/02/15
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Ghassemi B, Hosseini R, Dehghan nayeri F. The Effect of Nano Cobalt and Nano Chitosan on Artemisinin production and expression of SQS and DBR2 genes in Artemisia annua. Genetic Engineering and Biosafety Journal 2015; 4 (1) :25-39
URL: http://gebsj.ir/article-1-89-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 1 (4-2015) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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