fa به کارگیری روش های مختلف تأیید انجام واکنش RT-LAMP جهت تشخیص ویروس پیچیدگی برگ سیب زمینی (PLRV) مهندسی ژنتیک گیاهی Plant پژوهشي Research <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیماری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیچیدگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیماری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مهم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مزارع</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باعث</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کاهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عملکرد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیفیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">غده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود.</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیماری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توسط</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ویروس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیچیدگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایجاد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یکی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روشهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اولیه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مدیریت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آلودگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محصولات</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">غده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ویروس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به عنوان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">غده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بذری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کاشت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است.</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">متعددی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تشخیص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ویروس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سرولوژیک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جدیدی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تشخیص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پاتوژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></strong> <strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">RT- LAMP</span></span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مولکولی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اشاره</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کرد.</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تشخیص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ویروس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیچیدگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منظور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انجام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سرولوژیک،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نمونه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">علایم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشابه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ویروس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیچیدگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سیب</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سطح</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زنجان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جمع آوری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شدند</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انجام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آزمون</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مولکولی،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آر</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ان</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ا کل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استخراج</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش </span></span></strong>&nbsp;<strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">RT-LAMP</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> صورت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سپس</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلفی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تأیید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انجام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شدند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مثبت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دیدن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کدورت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حاصله،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بارگذاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محصول</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آگارز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رنگ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فلورسنت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سایبرگرین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اتیدیوم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بروماید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تأیید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد.</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مزایای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جدید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مقایسه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سایر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رو</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیشین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سرعت(</span></span></strong> <strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:14.0pt;">75</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دقیقه)،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سهولت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایمنی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اشاره</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کرد</span></span></strong><strong><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:14.0pt;">.</span></span></span></strong><br> &nbsp; <div>Potato leaf roll virus is an important virus that causes economic loss in the yield and quality of potato tubers. One of the primary methods of managing infection in potato crops is using certified virus-free tuber as &lsquo;seed&rsquo; for planting. Early and efficient detection of virus is essential for production of PLRV infection free tubers. There are several techniques to detect the virus including serological test and molecular methods. LAMP is a new method to identify pathogens that is used in this study for detection of potato leaf roll virus. Potato plants with symptoms similar to PLRV were collected from Zanjan province and were subjected to a serological test. Total RNA was extracted and RT-LAMP reactions were carried out. Different methods were used to confirm performance of this reaction. Positive reaction was confirmed based on the resulting turbidity and loading product on agarose gel and using SYBR and ethidium bromide florescence dyes. The advantages of this new method include the speed (75 min), ease and safety of the method compared to other methods.</div> رنگ های فلورسنت, کدورت, واکنش RT-LAMP, واکنش RT-PCR, ویروس پیچیدگی برگ سیب زمینی (PLRV) Florescence dyes, Potato Leaf Roll Virus (PLRV), RT-LAMP Reaction, RT-PCR, Turbidity 1 8 http://gebsj.ir/browse.php?a_code=A-10-172-3&slc_lang=fa&sid=1 Ms mohammadamin almasi محمد امین الماسی aminalmasi66@gmail.com 2929744227 10031947532846001720 Yes علی حق نظری ahaghnazari@gmail.com 10031947532846001721 10031947532846001721 No ابوبکر مرادی moradibio@gmail.com 10031947532846001722 10031947532846001722 No اسماعیل صابرفر 10031947532846001723 10031947532846001723 No