fa بهینه سازی محیط کشت تولید باکتری بومی گرمادوست Cohnella sp. A01، خالص سازی آنزیم کیتیناز و بررسی فعالیت قارچ کشی آن مهندسی ژنتیک گیاهی Plant پژوهشي Research <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جزء</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اصلی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کوتیکول</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حشرات</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پوسته</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سخت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پوستان</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بوده</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دیواره</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سلولی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیشتر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قارچ</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">، </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بعضی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جلبک</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نماتدها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تشکیل</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دهد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتینازها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هایی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هستند</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نقش</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تجزیه</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نامحلول</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عهده</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارند.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باکتری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برای</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هضم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کنند</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">،</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اصولا</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عنوان</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منبع</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کربن</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انرژی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کنند. در</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حداکثر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پنج</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نوع</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محیط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سویه</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بومی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایران&nbsp; </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(</span></span></span></span><em><span dir="LTR"><span style="font-size:12.0pt;">Cohnella sp. A01</span></span></em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توانایی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارد</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">، خالص سازی </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باکتری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بعد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اولیه</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محیط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیش</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منتقل</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بعد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گذشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">24</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ساعت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باکتری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به پنج</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محیط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مختلف</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">همگی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حاوی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کلوئیدی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عنوان</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جزء</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اصلی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بودند،</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">جهت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منتقل</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیشترین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مربوط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تیماری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بود</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شامل </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:timesnewromanps-boldmt,serif;"><span style="font-size:13.0pt;">(NH₄)₂SO₄</span></span></span></span> &nbsp;<span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بود</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سنجش</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فعالیت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کمک</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">معرف</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رنگی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">3</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">5</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نیترو</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سالیسیلیک</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اسید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رسوب</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دهی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پروتئین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توسط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آمونیوم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سولفات</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">انجام</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بعد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">رسوب</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دهی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پروتئین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آمونیوم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سولفات</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">میزان</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">فعالیت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیمی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">افزایش</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یافت</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حداکثر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عملکرد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باکتری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">طرح</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:timesnewromanps-boldmt,serif;"><span style="font-size:13.0pt;">SAS</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برای</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پنج</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نوع</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محیط</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تولید</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کمک</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نرم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">افزار</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کاملاً</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تصادفی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اثر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مهارکنندگی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نسبتاً</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خالص</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سازی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شده</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تعدادی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قارچ</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هایی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">عوامل</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیماری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زای</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">محسوب</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">می</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شوند</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نیز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اثر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مهارکنندگی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">(ضد</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قارچی)</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنزیم</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کیتیناز</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">روی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">چهار</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قارچ</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بیماری</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">زای</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گیاهی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ثابت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></span><br> &nbsp; Chitin, poly-&beta;-1,4-N-acetyl-D-glucosamine (GlcNAc), is the second most abundant organic polymer in nature after cellulose and it is the main part of insects cuticle and crustaceans that includes in cell walls of most fungi and some algae and nematodes. One of the enzymes that are responsible for disintegration of chitin is Chitinase. Bacteria produce Chitinase for digesting chitin, principally as a source of carbon and energy. With the objective of achieving the maximum production of Chitinase in an Iranian thermophile strain of <em>Cohnella sp</em>. A01, five different media were examined. We partially purified this enzyme from a native strain. Bacteria were transferred to a pre-culture media after an initial culture. After 24 hours, the bacteria were transferred to five different media containing colloidal chitin as the main component. Maximum enzyme production reached in a treatment that contained (NH4)2SO4 0.05% and Agar 0.1%. We performed Chitinase assay using the DNS (dinitrosalicylic acid) and precipitated proteins by Ammonium sulfate. After precipitation of proteins with Ammonium sulfate, enzyme activity was increased. Completely Randomized Design was used and statistical analysis was performed using SAS software. Anti-fungal effect of Chitinase partially purified in this study was tested on five plant fungal pathogens and was confirmed in four cases.<br> &nbsp; اثرات ضد قارچی, خالص سازی آنزیم, فعالیت آنزیمی, کیتیناز Chitinase, Enzyme Activity, Purification of Enzyme, Anti-Fungal Effect. 15 22 http://gebsj.ir/browse.php?a_code=A-10-251-1&slc_lang=fa&sid=1 Naghmeh Abiri نغمه عبیری 10031947532846001726 10031947532846001726 No Saeed Aminzadeh سعید امین زاده aminzade@nigeb.ac.ir 10031947532846001727 10031947532846001727 Yes Mohammad Reza Bihamta محمدرضا بی همتا mrghanad@ut.ac.ir 10031947532846001728 10031947532846001728 No