fa بررسی مولکولی وضعیت تراریختگی دانه های ذرت وارداتی به ایران مهندسی ژنتیک گیاهی Plant پژوهشي Research <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">حاضر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منظور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بررسی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وضعیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریختگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ذرت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وارداتی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مولکولی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">صورت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">است</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">همین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">منظور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">5</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نمونه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ذرت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وارداتی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نام های </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">PREVENTER</span></span></span></strong> <strong><span style="font-family:bzarbold;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">INCEILGAZ </span></span></span></strong><strong><span style="font-family:bzarbold;"><span style="font-size:12.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IRON LINDREW </span></span></span></strong><strong><span style="font-family:bzarbold;"><span style="font-size:12.0pt;">،</span></span></strong> <strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">AGIOS SOSTIS </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">MASTROGIORGI</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آرژانتین</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بندر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">امام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خمینی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ماهشهر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گمرک</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشور دریافت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;"> . </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آغازگرهای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اختصاصی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برای</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نواحی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تنظیمی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیشبر </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CaMV 35S</span></span></span></em></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> و پایان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دهنده </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">nos</span></span></span></em></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> طی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">واکنش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">سی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آر</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وضعیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بودن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">یا</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نبودن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نمونه های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مذکور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد بررسی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">قرار</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گرفت</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">&nbsp;در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اینورتاز</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنوم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تمام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ارقام</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ذرت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به عنوان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کنترل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داخلی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">استفاده</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">. </span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نتایج</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نشان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">داد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">که</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ذرت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وارداتی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">از</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کشور</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آرژانتین تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هستند</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ژنوم</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">خود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نواحی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تنظیمی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیشبر</span></span></strong> &nbsp;<strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CaMV 35S</span></span></span></em></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">و</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پایان</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دهنده </span></span></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">nos</span></span></span></em></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">را</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دارند</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong><strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;"> با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">توجه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پیوستن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایران</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">به</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">پروتکل</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ایمنیزیستی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">کارتاهنا،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">باید</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ثبت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشخصات</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مواد گیاهی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وارداتی،</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">وضعیت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریختگی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنها</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشخص</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">شود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اما</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اسناد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">همراه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">با</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ذرت وارداتی</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">هیچ</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">گونه</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">اظهاری</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مورد</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">تراریخته</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">بودن</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">این</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">دانه ها</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">در</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">برگه های</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">ثبت</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">مشخصات</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">آنها وجود</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:14.0pt;">نداشت</span></span></strong><strong><span dir="LTR"><span style="font-family:bzarbold;"><span style="font-size:14.0pt;">.</span></span></span></strong> This study was carried out to detect the presence of genetically modified maize in imported into Iran using molecular approaches. Five samples of imported maize from Argentina in the second half of 2010 were obtained from Bandar Imam Khomeini custom. Using specific primers for <em>CaMV 35S </em>promoter and <em>nos </em>terminator, PCR was performed. In this study <em>Invertase </em>gene of maize was used as internal control. The results showed that maize samples imported from Argentina were genetically modified and they have regulatory regions of <em>CaMV 35S </em>and <em>nos </em>in their genome. The shipment was not labled and there was no indication in the accompanying documents that the<br> shipment &ldquo;may contain living modified organisms&rdquo;. ایمنی زیستی, پروتکل کارتاهنا, ذرت, گیاهان تراریخته, واردات Biosafety, Cartagena Biosafety Protocol, Maize, Transgenic Plants, Imports 103 112 http://gebsj.ir/browse.php?a_code=A-10-257-1&slc_lang=fa&sid=1 Leila Sarmadi لیلا سرمدی 10031947532846001709 10031947532846001709 Yes Abbas Alemzadeh عباس عالم زاده alemzadeh@shirazu.ac.ir 10031947532846001710 10031947532846001710 No Behzad Ghareyazie بهزاد قره یاضی ghareyazie@yahoo.com 10031947532846001711 10031947532846001711 No