Promoter sequences are one of the key factors in directed gene expression. Different types of promoters can be used according to the type of tissue or expression levels desired. β-Conglycinin is one of the major storage proteins in soybean seed embryos and is produced in the seed development stage. The synthesis of storage proteins is primarily controlled at the transcriptional level and seed-specific expression has been shown to be conferred upon the promoter regions of many storage protein genes. In this research, a seed specific promoter (β-Conglycinin) of Iranian Glycin max was isolated from genomic DNA. Then, the promoter was cloned into pTZ57R/T and sequenced. Sequence analysis showed that the cloned promoter contained all of the typical conserved motifs such as TATA box, CAAT box as well as other previously identified seed-specific motifs such as SKn-1, RY repeats, G box. Finally, the β-Conglycinin promoter was cloned into pBI121 binary vector for further research.