biotechnology needs the genetic resources to establish the sustainable tolerance to salinity stress in GMO plants. Aleluropus littoralis is known as a good model plant for understanding the genetic and molecular mechanism of salt resistance in monocots. This plant has a high genetical homology with rice. In this study, 150 ESTs were isolated from A. littoralis. Firstly, repeated sequences were eliminated and then the possible function and similarity of the remained ESTs were compared with other ESTs of different plants using international genetic databases. Results of this study showed that, 71 of the selected ESTs were similar to the ESTs in the databases and their function have been determined. Moreover, 14 ESTs were similar to the ESTs in the databases but their functions have not been determined yet. Seven ESTs did not show any similarity. Alignment analysis showed that the isolated ESTs were categorized in 19 different groups. 16 ESTs acted as a transporter, 12 ribosomal proteins and the other ESTs were related to channel transfer regulators, RNA editing and cell division. By the relatively high level of homology with rice genes, the genetic studies on Aleuropus could help in determining the differences in salt-tolerance between glycophyte and halophyte grass