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:: Volume 7, Issue 1 (8-2018) ::
2018, 7(1): 33-40 Back to browse issues page
Effects of Agrobacterium rhizogenes strains and explants on induction of hair root in Physalis alkekengi
Arezoo Aziz khajeh , Ebrahim Dorani * , Saied Aharizad
Plant Breeding and Biotechnology Dept., Agriculture Faculty, Tabriz University, Tabriz, Iran
Abstract:   (222 Views)
Physalis alkekengi L. is a medicinal plant belonging to the Solanaceae family. This plant is rich in phytochemicals such as: physalins, withanolides, sterols, polysaccharides and flavones. P. alkekengi has many uses in traditional medicine and pharmaceutical industry. Hairy root cultures of P. alkekengi can be used to produce secondary metabolites. In this research, the effects of 5 strains of Agrobacterium rhizogenes (GM, C58, A4, MSU and 15834) and leaf and stem explants were studied on induction of hairy root in P. alkekengi. The highest transformation rate was related to explants which were inoculated with A4, MSU and 15834 strains. Leaf explants produced 5.6 hairy roots per explant and were better than stem explants (produced 2.9 hairy roots per explant). To confirm the transformation of hairy roots, PCR was performed with specific primers of rol A gene. Electrophoresis of PCR products confirmed the integration of rol A gene (403 bp) to the plant genome. This article is a first published study about induction of hairy root in P. alkekengi.
Keywords: Agrobacterium rhizogenes, genetic engineering, hairy root, medicinal plant, Physalis alkekengi
Full-Text [PDF 790 kb]   (46 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2018/02/9 | Accepted: 2018/08/17 | Published: 2018/09/3
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Aziz khajeh A, Dorani E, Aharizad S. Effects of Agrobacterium rhizogenes strains and explants on induction of hair root in Physalis alkekengi. Genetic Engineering and Biosafety Journal. 2018; 7 (1) :33-40
URL: http://gebsj.ir/article-1-238-en.html


Volume 7, Issue 1 (8-2018) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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