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:: Volume 7, Issue 1 (8-2018) ::
2018, 7(1): 51-60 Back to browse issues page
The reaction of transgenic rice plants containing EPSPS gene to glyphosate
Sedigheh Nasr Ramzi , Mohammad Mahdi Sohani , Reza Shirzadian Khorramabad , Mahyar Amininasab , Jafar Asghari
Plant Biotechnology Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Abstract:   (79 Views)

The most effective way to produce a plant resistant to glyphosate is manipulation of EPSPS enzyme inorder to reduce its affinity to this herbicide. It is one of the vital enzymes in the shikimate pathway, which is responsible for producing aromatic amino acids and various secondary metabolites in plants and bacteria. Without an active enzyme, the organism will not be able to survive. In the present study, one of the important domains in the EPSPS enzyme modified by Site-directed mutagenesis using megaprimer method and one tube approach. Glycine in position 96 modified to Alanine which was sub-cloned along with the wild-type gene into pPZPY122 plant binary vector. Hashemi cultivar was used for transformation using EHA105 strain of Agrobacterium tumefaciens with in planta method. T2 transgenic plants separately were evaluated with resistance to the gentamicin and glyphosate. Also, positive gene expression was confirmed by Real Time Quantitative PCR. Therefore, based on the results, these plants showed significantly a high degree of resistance to the herbicide.
 

Keywords: Gene expression, Glyphosate, In planta transformation, Rice, Herbicide
     
Type of Study: Research | Subject: Plant
Received: 2018/07/4 | Accepted: 2018/10/7 | Published: 2018/10/7
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Nasr Ramzi S, Sohani M M, Shirzadian Khorramabad R, Amininasab M, Asghari J. The reaction of transgenic rice plants containing EPSPS gene to glyphosate . Genetic Engineering and Biosafety Journal. 2018; 7 (1) :51-60
URL: http://gebsj.ir/article-1-258-en.html


Volume 7, Issue 1 (8-2018) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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