Designing and Constructing of Nano Biosensor Based on Grafen Oxide Nano Particles for Detection of Grapevine Fanleaf Virus
|
Fatemeh Shokri , Mehrdad Salehzadeh * |
Plant Pathology, Faculty of Agriculture, University of Tabriz, Iran , mehrdadsalehzadeh@gmail.com |
|
Abstract: (4537 Views) |
The most destructive viral disease in vine plants is grapevine fan leaf virus (GFLV) which causes severe damages to vineyards every year. Since the area under grape cultivation is significant compared to other crops and its propagation is done by vegetative materials, therefore, the identification of this disease in vineyards using a rapid method with high sensitivity is essential. In this study, in order to design a nano-biosensor based on graphene oxide, cDNA of the coat protein (CP) gene of GFLV was used. The basis of action of this sensor is the fluorescence emission obtained from the DNA probe. If the probe DNA is hybridized with the cDNA of the CP gene, the emission fluorescence is observed and measured using a fluorimetric device. In order to optimize the nanosensor, all factors of binding time of DNA probe to graphene oxide, graphene oxide concentration, time of hybridization of cDNA to DNA probe and concentration of cDNA for hybridization with probe attached to graphene oxide were evaluated. The results showed that the designed nanosensor was able to detect CP gene and could detect GFLV with high efficiency up to 10-13 mol/l. |
|
Keywords: Desin and Construction of Nano Biosensor Based on Grafen Oxide Nano Partical for Detection of Grapvine Fanleaf Virus(GFLV) |
|
Full-Text [PDF 1044 kb]
(1452 Downloads)
|
Type of Study: Research |
Subject:
Microrganisms and Viruses Received: 2019/07/21 | Accepted: 2019/12/6 | Published: 2020/02/3
|
|
|
|
|
Add your comments about this article |
|
|