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Assessment of relative gene expression of wdhn5 in wild and agronomy wheat genotypes under drought stress conditions
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Homeyra Shahi   , Nafise Mahdinezhad , Fatemeh Haddadi , Bahman Fazeli nasab * |
| Research Department of Agronomy and Plant Breeding, Agriculture Research Institute, University of Zabol, Iran , bfazelinasab@gmail.com |
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Abstract: (4288 Views) |
In order to examine the role of the wdhn5 regulatory factor and its expression pattern as well as the physiological characteristics during drought stress in wheat plant, a factorial experiment was carried out in completely randomized design in pot with three genotypes. First factor was the wheat genotypes including two hexaploide cultivars (Bolani, Sistan), two tetraploide cultivars (Behrang, Shabrang) and wild types (Urartu, Tauschii, Speltoides). The irrigation treatment with three levels consisted of normal (90% of field capacity), mild stress (60% of field capacity) and severe stress (40% of field capacity) was as the second factor. Analysis of variance for physiological characteristics showed that the effect of water stress on all traits were significant, and the sensitive cultivar Speltoides had significantly lower dry mater production, water use efficiency and relative water content than the other cultivars. The result of real time PCR on relative expression of wdhn5 gene showed that its expression in Tauschii and Bolani tolerant cultivars in 40% of field capacity increased significantly which could indicate the importance of this gene in drought stress conditions. Based on these results, Bolani cultivar and Tauschii wild cultivar are relatively tolerant to drought and Splotheids is sensitive to drought.
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| Keywords: Field Capacity, water use efficiency, Dry matter, relative water content |
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Full-Text [PDF 882 kb]
(1022 Downloads)
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Type of Study: Research |
Subject:
Plant
Received: 2019/09/26 | Accepted: 2020/01/5 | Published: 2020/02/4
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