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:: Volume 11, Issue 1 (4-2022) ::
2022, 11(1): 85-96 Back to browse issues page
Investigation of transformation and expression of roseltide rT1 gene in wheat
Shokoufeh Abdolmajidi , Fatemeh Dehghan Nayeri *
Biotechnology Department, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran. , nayeri@eng.ikiu.ac.ir
Abstract:   (999 Views)
Antimicrobial peptides display powerful antimicrobial activities against a large set of plant pathogens and are a good alternative to conventional toxins. To transfer rT1 gene of Hibiscus sabdariffa to wheat, Agrobacterium tumefaciens strain LBA4404 harboring the pBI121-rT1 plasmid was used. Mature embryo explants of four wheat cultivars including the Saison, Bahar, Chamran and Arta after co-cultivated with A. tumefaciens suspension were transferred to a selective callus medium containing 2 mg/l 2,4-D, 2 mg/l Picloram, 50 mg/l Kanamycin and 500 mg/l Cefotaxime. Embryogenic calli were selected after growth in a selective medium and then transferred to a regenerative medium containing 25 mg/l Kanamycin and 250 mg/l Cefotaxime. The presence and expression of rT1 gene at the transcriptional level in transgenic plants were confirmed by PCR and RT-PCR methods respectively using specific primers. Based on the RT-PCR results, the rT1 gene was expressed in transgenic wheat plants. In this study, out of 15 possible transgenic plants, only 11 were transgenic. Among transgenic plants, five plants belonged to Saison cultivar with 1.6 transgenic percentage, four plants represented to the Bahar cultivar with 1.3 transgenic percentage and two plants belonged to the Chamran cultivar with 0.6 transgenic percentage. No transgenic plant was obtained for the Arta cultivar.
Article number: 8
Keywords: Agrobacterium, Antimicrobial peptides, rT1 gene, RT-PCR, Transgenic wheat
Full-Text [PDF 1126 kb]   (135 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2022/06/11 | Accepted: 2022/08/28 | Published: 2022/09/17
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Abdolmajidi S, Dehghan Nayeri F. Investigation of transformation and expression of roseltide rT1 gene in wheat. Genetic Engineering and Biosafety Journal 2022; 11 (1) : 8
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Volume 11, Issue 1 (4-2022) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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