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Evaluation of genetic variation in doubled haploid lines of Camelina using REMAP retrotransposon markers
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Hamzeh Minaei Chenar    , Sajad Rashidi Monfared * , Danial Kahrizi , Leila Zarei , Amin Ebrahimi |
| Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Thran, Iran , rashidims@modares.ac.ir |
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Abstract: (1429 Views) |
Iran is an arid and semi-arid country and more than 70% of its agricultural lands are rainfed. So far, no oilseed plant suitable for different regions of the country has been introduced. Camelina (Camelina sativa L.) is an oily-medicinal plant of the Brassicaceae family, a low-input plant, with low water-fertilizer requirements, resistant to a variety of common diseases and pathogens and suitable for cultivation in grain rotation. In this study, genetic variation of 32 doubled haploid lines of Camelina was evaluated by using 11 REMAP primers. Eight primers produced clear and scorable strips and totally, 74 bands were produced which 59 of them were polymorphic )79/72 %). IRAP1+P6 and IRAP1+P8 primers produced the lowest number of bands (4 bands) and IRAP979+P4 marker produced the most of bands (15 bands). The amount of primer polymorphism varies from 23.69% to 100% and the amount of PIC varies from 0.38 to 0.41. Clustering based on the Jaccard similarity placed the lines in six main groups. According to the principal components analysis, the first two components contributed 21.51% of the variance, which indicated the appropriate coverage of the markers at the genome level. Thus, it seems that the REMAP marker, with its high polymorphism and high distribution in the Camelina genome, is a suitable marker for investigating the genetic diversity in this plant. |
Article number: 6 |
| Keywords: Camelina, genetic variation, PCR, primer, REMAP marker |
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Full-Text [PDF 930 kb]
(249 Downloads)
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Type of Study: Research |
Subject:
Divers
Received: 2022/07/31 | Accepted: 2022/10/3 | Published: 2022/11/27
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