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Preservation of viability and virulence of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae by formulation in an oil invert emulsion
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Ali Mehrvar *    , Parya Soleimani , J.P. Michaud* , Nahid Vaez |
| Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran , a.mehrvar72@yahoo.com |
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Abstract: (1365 Views) |
Using Ephestia kuehniella larvae as hosts, invert emulsion (IE) and an aqueous suspension (AS) of Beauveria bassiana (isolate IR34-JS2) and Metarhizium anisopliae (isolate IR41-TT1) formulations were compared when stored for three months at either 3 °C or 25 °C. Initially, IE formulations of isolates JS2 and TT1 were 13- and 14-fold more virulent than AS formulations, based on their LC50 values. JS2 LC50 values increased 1.3- and 5.6-fold for the AS and IE formulations, respectively, after 3 months storage at 3 °C, and 1.6 and 7.3-fold after 3 months at 25 °C, whereas values for TT1 increased 2.1- and 6.8-fold for these formulations at 3 °C, and 3.9- and 15.7-fold at 25 °C. Formulation and storage temperature interacted significantly to affect conidial production post-storage. Vegetative growth, conidiogenesis, and conidial germination of both isolates were better preserved at 3 °C than at 25 °C in vitro. The IE formulation preserved vegetative growth capacity better than the AS formulation at both temperatures, and better preserved conidiogenesis at 3 °C, a temperature that also improved TT1 conidial germination in the IE formulation. At 3 °C, the IE formulation yielded a relative speed of kill that was 3.5- and 1.3-fold faster for the JS2 and TT1 isolates, respectively, than the AS formulation. It can be concluded that IE formulations were superior to AS formulations, and that storage at 3 °C will help preserve the biological activity and pathogenicity of these fungi. |
Article number: 2 |
| Keywords: Beauveria bassiana, Ephestia kuehniella, Metarhizium anisopliae, fungal bioindices. |
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Full-Text [PDF 825 kb]
(303 Downloads)
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Type of Study: Research |
Subject:
Biosafety
Received: 2022/07/30 | Accepted: 2022/08/31 | Published: 2022/10/28
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