CRISPR/Cas Systems Diversity in Leuconostoc Genus

Ghaffarian, Sara; Panahi, Bahman

doi:10.61186/gebsj.12.2.281

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Volume 12, Issue 2 (11-2023)

gebsj 2023, 12(2): 281-293

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CRISPR/Cas Systems Diversity in Leuconostoc Genus

Sara Ghaffarian ^*

, Bahman Panahi

Department of Cellular and Molecular Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran , s.ghaffarian@azruniv.ac.ir

Abstract: (1057 Views)

Bacteriophage infections are one of the key challenges in the use of various bacteria in industries. CRISPR-Cas systems are part of the bacterial immune system against viral infections and determining the characteristics of these systems can be beneficial for the formulation and use of these bacteria. To investigate the CRISPR/Cas system in the Leuconostoc genus, the complete genome sequences of 18 reported species of this genus were explored and their CRISPR/Cas system information was analyzed using homology-based algorithms. Then, the characteristics of the conformational structure and stability of recognized systems were determined based on MFE values, phage recognition sites were identified using BLAST approaches, and the evolutionary relationships of these systems were analyzed based on amino acid sequence of associated proteins. Based on the obtained results, 9 of 18 reported species for this genus contain whole CRISPR/Cas system. CRISPR/Cas type analysis confirmed that except for one case, all strains contained the type II-A system. In these systems, the number of repeats and average length of spacers varied from 4 to 101 and 28 to 30 nucleotides, respectively. Nine types of PAM sequences were identified at the 3´ and 5´ ends of these systems. Based on relationship analysis, the studied system was divided into two main groups. Subtype II-A appears to be the most active system against foreign DNA and phages in the Leuconostoc genus.

Keywords: Leuconostoc, CRISPR, Diversity, Relationship, Structure.

Full-Text [PDF 1400 kb] (252 Downloads)

Type of Study: Research | Subject: Divers
Received: 2023/08/2 | Accepted: 2024/02/13 | Published: 2024/03/14

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