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Induction of hairy roots in multi-year-old yew (Taxus baccata) seedlings using Agrobacterium rhizogenes for the production of taxol
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Mahdi Mahmoudi    , Seyed Ahmad Sadat Noori * |
| Department of Agronomy and Plant Breeding, Abu Raihan Faculty of Agricultural Technology, University of Tehran, Pakdasht, Iran , noori@ut.ac.ir |
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Abstract: (44 Views) |
Yew (Taxus baccata) is a diploid plant with a chromosome number of 2n=2x=24, belonging to the order Taxales and the family Taxaceae. Among its most important secondary metabolites is taxol, a well-known anticancer drug. The aim of this study was to investigate the induction of hairy roots in perennial yew seedlings using Agrobacterium rhizogenes to optimize the production of secondary metabolites under in vitro conditions. The experiment was conducted as a factorial design in a completely randomized format with three replications. The factors examined included explant type at two levels (root and leaf) and bacterial strain at three levels (ATCC-15834, TR107, MAFE01724). The results demonstrated that the interaction of the examined factors significantly influenced the traits of hairy root induction percentage, fresh weight, and dry weight of hairy roots after two months. The highest percentage of hairy root induction, as well as fresh and dry weights, were achieved with the combination of the MAFE01724 strain and leaf explants, which were significantly higher compared to other treatment combinations. The transgenic nature of the hairy roots was confirmed by tracing a segment of the rolB gene using PCR amplification, while no corresponding band was amplified for non-transgenic (wild-type) roots. Overall, the results of this study indicate that the induction of hairy roots in perennial yew (Taxus baccata) seedlings is influenced by the explant type and bacterial strain. These findings provide an essential prerequisite for further experiments on hairy root cultures aimed at the production of pharmaceutical metabolites.
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Article number: 9 |
| Keywords: Yew, Hairy root, Agrobacterium rhizogenes, Taxol |
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Type of Study: Research |
Subject:
Plant
Received: 2025/02/27 | Accepted: 2025/08/3 | Published: 2025/09/14
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