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Comparison of the use of chemical and organic disinfectants for disinfection of Syngonium podophyllum L. varieties under in vitro conditions
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Mina Taghizadeh *    , Mahsa Aghili-Gavari |
| Department of Horticultural science, Faculty of Agriculture and environmental science, Arak University, Arak, Iran , m-taghizadeh@araku.ac.ir |
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Abstract: (604 Views) |
Syngonium podophyllum L. ornamental plant is widely grown as a houseplant due to its diversity and tolerance to low light conditions. In addition to being unsuccessful, the use of conventional disinfectants may cause toxicity to the explants and in most cases cannot control all microorganisms. Attention has been paid to new alternative disinfectants. This study investigated disinfection with ethanol and household bleach, tobacco extract, cumin essential oils (20, 100 and 200 mg L-1), antibiotics (cefazolin, chloramphenicol, streptomycin and penicillin) and different application methods (in culture medium or immersion) for Syngonium podophyllum varieties in several experiments. The results showed that the in vitro contamination depends on the varieties, method and type of disinfectant. Conventional disinfection methods were not effective in eliminating bacteria. Supplementing the culture medium with 20 mg L-1 of cumin essential oil effectively inhibited bacterial growth in the Emerald Green variety. Meanwhile, cefazolin at 100 mg L-1 in the medium successfully eradicated both bacterial contamination in Emerald Green and fungal contamination in the Christmas variety. Furthermore, disinfecting explants with tobacco extract for 30 minutes completely eliminated bacterial and fungal contamination across the Christmas and Emerald Green varieties. These findings suggest the potential use of compounds such as cumin and tobacco as cost-effective and sustainable disinfection alternatives, provided that the type of endogenous contamination is accurately predicted.
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| Keywords: antibiotic, bacteria, micropropagation, fungi, disinfection |
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Full-Text [PDF 841 kb]
(82 Downloads)
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Type of Study: Research |
Subject:
Biosafety
Received: 2026/04/22 | Accepted: 2026/06/22 | Published: 2026/06/22
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