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:: Volume 6, Issue 1 (7-2017) ::
2017, 6(1): 49-63 Back to browse issues page
In silico study of polyamine oxidase (PAO) gene family in grape
Amin Abedi , Mohammad Mehdi Sohani , Reza Shirzadian-Khoramabad
Department of Agricultural Biotechnology, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
Abstract:   (420 Views)

Growth, development and productivity of plants are greatly affected by various abiotic stresses. Physiological and molecular studies have shown that naturally occurring plant polyamines (PAs) are involved in conferring abiotic stress tolerance in plants. Polyamine oxidases (PAOs) are FAD-dependent enzymes associated with polyamine catabolism in peroxisomes, the apoplast and the cytoplasm. In plants, increasing evidence supports the idea that PAO genes play essential roles in abiotic and biotic stresses responses. In this study,  bioinformatic analysis identified eight putative PAO genes (VvPAO1–VvPAO8) in grape (Vitis vinifera) using the released 12×assemble grape genomic sequences. Phylogenetic analysis indicates that these VvPAOs can be classified into three subgroups as found in Arabidopsis and rice and also reveal that grape PAO proteins are more closely related to Arabidopsis than to those in rice. The VvPAO genes contained zero to nine introns and were distributed across 6 out of the 19 chromosomes in grape. Promoter analysis showed the presence of several cis-regulatory elements related to stress and hormone responses in regulatory regions, indicating their probable role in stress response. Microarray-based expression analysis of VvPAO orthologs in Arabidopsis under abiotic stresses showed that transcript levels of PAO genes were up-regulated significantly by such treatments, indicating their vital roles during stress adaptation. The results obtained provide basic information for future research on the functions of PAO genes in grape.

Keywords: Abiotic Stress, Bioinformatics, Gene Expression, Phylogenetic Analysis, Polyamine
Full-Text [PDF 423 kb]   (50 Downloads)    
Type of Study: Research | Subject: Divers
Received: 2017/01/13 | Accepted: 2017/07/10 | Published: 2017/08/10
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Abedi A, Sohani M M, Shirzadian-Khoramabad R. In silico study of polyamine oxidase (PAO) gene family in grape . Genetic Engineering and Biosafety Journal. 2017; 6 (1) :49-63
URL: http://gebsj.ir/article-1-161-en.html
Volume 6, Issue 1 (7-2017) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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