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Study of genetic diversity and phylogenetic relationship of some gourd pumpkin (Cucurbita pepo) genotypes using the ITS ribosomal and rbcL chloroplastic genes’ loci
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Marzieh Sheikhi   , BaratAli Fakheri * , Nafiseh Mahdinezhad |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Iran , fakheri@uoz.ac.ir |
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Abstract: (4396 Views) |
| The method of DNA barcoding has a great potential for the revival of archeology and traditional biodiversity studies; therefore, this method has so far been widely used in studies on plants and for purposes of classification and archeology. In this research, 96 pumpkin (Cucurbita pepo) plants from eight genotypes were planted in greenhouse according to a completely randomized design, and young leaves were harvested from samples at 4-leaf stage. In the next step, DNA extraction, its quantitative and qualitative evaluation, PCR and sequencing were carried out and data analysis was performed using Clustalw2 and BioEdit software. The results showed that in the nucleotide sequences of the ITS gene in the pumpkin family, cytosine (29.5) was highest and adenine (20.3) was the least frequent and in rbcL gene, adenine (27.4) was highest and cytosine (20.1) was the least frequent. Estimation of the nucleotide replacement of rbcL and ITS gene in the pumpkin family had high levels of pyrimidine replacement, resulting in the conversion of thymine to cytosine 31.99% and 20.43%, respectively, and for the conversion of cytosine to thymine was 47.05 and 16.04%, respectively. The primers of ITS identified only five genotypes while rbcL identified all eight genotypes, so the rbcL primers is more suitable for sequencing. |
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| Keywords: DNA barcoding, ITS, rbcl, pumpkin |
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Full-Text [PDF 595 kb]
(1112 Downloads)
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Type of Study: Research |
Subject:
Plant
Received: 2019/07/28 | Accepted: 2019/12/6 | Published: 2020/02/3
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