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:: Volume 9, Issue 2 (10-2020) ::
2020, 9(2): 11-20 Back to browse issues page
Identification of soybean circular RNAs in response to low nitrogen and phosphorus stress
Saeid Babaei, Esmaeil Ebrahimie *, Ali Niazi, Mahnaz Nezamivand Chegini
Institute of Biotechnology, Shiraz University , ebrahimie@shirazu.ac.ir
Abstract:   (243 Views)
Soybean, one of the most important sources of edible oil and protein in the world, is exposed to various environmental biotic and abiotic stresses. These stresses can negatively impact the quality and quantity of soybean production. This study aimed to identify genes that express circular RNAs in response to low phosphorus and nitrogen stresses in soybean roots. Soybean seeds were grown under different low phosphorus and nitrogen treatments, and sampling was done from the root tissues for total RNA isolation. After measuring the quality and quantity of the isolated RNA, samples were sent to the Macrogen company (South Korea public biotechnology company) for next-generation sequencing. After obtaining the sequencing data, the quality of RNA-seq data measured, and then circular RNAs were identified and characterized using five different programs. Finally, to identify the expression pattern and putative function of circular RNAs, differential expression analysis and functional analysis of circular RNAs’ parent genes was done using bioinformatics approaches. RNA-seq experiment produced more than 360 million reads, and in total, 2033 circular RNAs were identified in all treatments. Although some circular RNAs were co-expressed in two or more treatments, most of the circular RNAs had a stress-responsive expression. Our results were revealed that circular RNAs may play their roles by binding to their target molecules. Identified genes can be used in plant breeding programs for producing tolerant soybean cultivars to low phosphorus and nitrogen stresses.
Keywords: Soybean, Stress, Phosphorus, Nitrogen, Circular RNA
     
Type of Study: Research | Subject: Plant
Received: 2020/08/23 | Accepted: 2020/11/10 | Published: 2020/11/15
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Babaei S, Ebrahimie E, Niazi A, Nezamivand Chegini M. Identification of soybean circular RNAs in response to low nitrogen and phosphorus stress. Genetic Engineering and Biosafety Journal. 2020; 9 (2) :11-20
URL: http://gebsj.ir/article-1-349-en.html


Volume 9, Issue 2 (10-2020) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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