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Effect of Pseudomonas flourescens FY32 bacteria on leaf proteome pattern of rapeseed under salinity stress
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Ali Bandehagh *   , Mahmoud Toorchi , Davoud Farajzadeh , Zahra Dehganian , Seddige Pirzad |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture,University of Tabriz, Iran , bandehhagh@tabrizu.ac.ir |
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Abstract: (4533 Views) |
| The ability of endophyte bacteria Pseudomonas flourescens FY32 in growth improving and increasing the resistance of rapeseed to salinity stress, through proteomic approach was investigated. This research was arranged in a factorial experiment on the basis of completely randomized design with four replications in hydroponic culture system. First factor was inoculation with bacteria and non- inoculation and the second factor was salinity stress in two levels (0 and 300 NaCl mM). The results showed that the effects of salinity stress, bacteria inoculation and canola cultivars were significant for dry weight, shoot and root (P< 0.01). The highest weight was related to the control and the lowest to severe stress. Inoculated plants had also the highest dry weight comparing to non-inoculated plants that indicates the positive effect of bacterial inoculation on canola in reducing the negative effects of salinity stress. Protein pattern of leaf tissue was determined by 2 dimensional electrophoresis. After scanning, second dimensional gels were analyzed through PD-quest software, then quality and quantity of protein expression in different treatment was compared. A total of 15 spots were significantly changed. Among them, four spots were related to oxidative stress, four spots were involved in metabolism pathway and seven spots were related to photosynthesis. Based on the comparison between bacteria and control gels under both stressed and non-stressed environments, the bacteria increased the tolerance of the plant under salinity stress. |
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| Keywords: Canola, PGPR, Salt stress, Two- dimensional electrophoresis |
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Full-Text [PDF 743 kb]
(1577 Downloads)
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Type of Study: Research |
Subject:
Divers
Received: 2019/01/7 | Accepted: 2019/03/17 | Published: 2019/04/20
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