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Viral vectors as a tool for gene transfer and the production of transgenic animals
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Zahra Roudbari *   , Khadijeh Nasiri |
| Department of Animal Science, Faculty of Agriculture, University of Jiroft, Iran , Roudbari.zahra@ujiroft.ac.ir |
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Abstract: (6591 Views) |
| Gene transfer technology is based on the use of animals and birds as bioreactors in the production of high-quality pharmaceutical, industrial and recombinant proteins, as well as the use of animal models for specific diseases and the investigation of the effects of therapeutic genes in them. Different methods have been developed for gene transfer in eukaryotic cells, including physical, chemical and viral methods. Technological advances and the ever-growing knowledge of molecular virology and virus-host cell relationships have improved the safety of viral vectors that are now used to study cellular gene function, to correct genetic defects (gene therapy), to express therapeutic proteins, to vaccinate against infectious agents and tumors, to produce experimental animal models, and for other purposes. By increasing the researchers' knowledge about the life cycle of viruses and due to their natural ability of viruses to transmit and integrate into the host genome, viruses have become one of the most powerful tools for transferring the gene. Viral vectors have been used in various forms for gene transfer and the production of transgenic animals, including the direct injection of recombinant viruses into the target tissue or the treatment of the stem cells with the recombinant virus and the transfer of recombinant cells to the target tissue or treatment of embryonic cells in the early stages of the fetus. In this study, we attempt to refer some of the most methods for gene transfer using viral vectors, their advantages and limitations in gene transfer. |
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| Keywords: Gene transfer, Transgenic animals, Viral vector, Recombinant virus |
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Full-Text [PDF 755 kb]
(16529 Downloads)
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Type of Study: Review |
Subject:
Animal
Received: 2019/01/7 | Accepted: 2019/03/17 | Published: 2019/04/20
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