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Induction and study of hairy root growth pattern in Indian ginseng medicinal plant (Withania somnifera.L) using Agrobacterium rhizogenes
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Nasibe Soltaninezhad *    , Seyed Ahmad Sadat-Noori , Ali Izady- Darbandi , Fateme Amini , Mohammad Hossein Mirjalili |
| Department of Agricultural Sciences and Plant Breeding, Aburaihan Faculty of Agricultural Technology, University of Tehran, Pakdasht, Tehran, Iran , soltaninezhad.na@ut.ac.ir |
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Abstract: (151 Views) |
Indian ginseng (Withania somnifera.L) is a compatible plant that has anti-cancer properties due to the presence of the active substance withanolide and is used to improve the overall health of the body and increase longevity. One of the important methods to increase secondary metabolites in medicinal plants is hairy roots culture. Hairy roots are genetically stable and able to increase metabolites without using hormones. In order to induce hairy roots, three different strains of Agrobacterium rhizogenes (A4, Atcc-15834, R318) were used. The percentage of root hair induction increased from A4 strain (66%) to Atcc-15834 strain (72%) and R318 strain (83%), respectively. The growth pattern of the strains was drawn and the peak growth of the strains was obtained in the fifth week. The doubling time of hairy roots was determined for the A4 strain on the fifth day, the Atcc 15834 strain on the ninth day, and the R318 strain on the 20th day. The maximum biomass produced was obtained in A4 strain (4.29 g), Atcc-15834 (2.38 g) and R318 (1.35 g). The design was implemented as a factorial experiment in the form of a completely randomized design with 3 replications. Data analysis was done with SAS 9.4 software and graphs were drawn with Excel. The results of analyzing the variance of wet and dry biomass weight of hairy roots showed that there is a significant difference between the studied strains at different times, and the obtained results correspond to the biosynthetic pathway of the plant.
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| Keywords: Agrobacterium rhizogenes, growth pattern, Withania somnifera.L, withanolide. |
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Type of Study: Research |
Subject:
Plant
Received: 2023/06/8 | Accepted: 2023/09/8 | Published: 2024/03/14
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