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:: Volume 12, Issue 2 (11-2023) ::
gebsj 2023, 12(2): 184-194 Back to browse issues page
Induction and study of hairy root growth pattern in Indian ginseng medicinal plant using Agrobacterium rhizogenes
Nasibe Soltaninezhad * , Seyed Ahmad Sadat-Noori , Ali Izady- Darbandi , Fateme Amini , Mohammad Hossein Mirjalili
Department of Agricultural Sciences and Plant Breeding, Aburaihan Faculty of Agricultural Technology, University of Tehran, Pakdasht, Tehran, Iran , soltaninezhad.na@ut.ac.ir
Abstract:   (974 Views)
Indian ginseng (Withania somnifera) is a plant adapted to the climatic conditions of the southern regions of Iran. One of the important methods to increase secondary metabolites in medicinal plants is hairy root culture. Hairy roots are genetically stable and able to increase metabolites without using hormones. In order to increase hair root production, three different strains of Agrobacterium rhizogenes (A4, Attc-15834, R318) were used. The percentage of hair root formation induction was determined as 66% in A4 strain, 72% in Atcc-15834 strain and 83% in R318 strain. The growth pattern of the strains was drawn and the peak growth of the strains was obtained in the fifth week. The doubling time of root hairs was determined for the A4 strain on the fifth day, the Atcc-15834 strain on the ninth day, and the R318 strain on the 20th day. The maximum biomass produced by strain A4 (4.29 g), Atcc-15834 (2.38 g) and R318 (1.35 g) was obtained. The results of analyzing the variance of wet and dry biomass weight of hairy roots showed that there is a significant difference between the studied strains in terms of hairy root induction at different times. The best strain for inducing the formation of hairy roots in Indian ginseng was determined as strain A4.

 
Keywords: Agrobacterium rhizogenes, growth pattern, Withania somnifera.L, withanolide.
Full-Text [PDF 912 kb]   (194 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2023/06/8 | Accepted: 2023/09/8 | Published: 2024/03/14
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Soltaninezhad N, Sadat-Noori S A, Izady- Darbandi A, Amini F, Mirjalili M H. Induction and study of hairy root growth pattern in Indian ginseng medicinal plant using Agrobacterium rhizogenes. gebsj 2023; 12 (2) :184-194
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Volume 12, Issue 2 (11-2023) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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