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The effect of Trichoderma harzianum and Bioguard as a commercial biological product against Rhizoctonia root rot of Chitti bean (Phaseolus vulgaris L.)
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Akbar Shirzad *   , Rogaie Habibi , Vahid Fallahzadeh , Vahid Sarabi |
| Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran , ashirzad98@yahoo.com |
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Abstract: (663 Views) |
Root rot and seedling blights of beans caused by Rhizoctonia solani is one of the most important fungal diseases in beans. In this study, the inhibitory effect of Trichoderma harzianum biocontrol isolate and Bioguard biological product was investigated in vitro and greenhouse conditions on the pathogenic fungus R. solani in 2020. The effect of treatments on disease inhibition, plant growth indices, chlorophylls and carotenoids content and peroxidase, phenylalanine-ammonialyase enzymes were studied. The results of in vitro assay showed that although Bioguard significantly prevented the growth of pathogenic mycelium in the culture medium, however, the treatment of Trichoderma harzianum showed more efficient with 51% inhibition rate. In greenhouse studies, both T. harzianum and Bioguard treatments had a significant effect on disease control. In terms of the effect on plant growth indicators, Bioguard had the highest stem and root fresh weight as well as stem and root length. In terms of chlorophyll content of bean leaves and carotenoids content, both Bioguard and Trichoderma treatment with pathogenic fungus R. solani showed a significant difference with the diseased control plant. Bioguard and Trichoderma antagonist fungus treatments together with pathogenic fungus led to the highest activity of phenylalanine ammonialyase and peroxidase enzymes and had statistically significant differences with Bioguard and T. harzianum alone treatments. Therefore, it is recommended to use both Trichoderma harzianum fungus and Bioguard as a commercial product in the occurrence of bean rhizoctonia root rot disease.
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Article number: 2 |
| Keywords: Antagonist, Biocontrol, Induced Resistance, Legumes, Physiological traits. |
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Type of Study: Research |
Subject:
Biosafety
Received: 2024/08/19 | Accepted: 2024/11/13 | Published: 2024/12/18
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