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:: Volume 13, Issue 2 (1-2025) ::
gebsj 2025, 13(2): 180-189 Back to browse issues page
Optimization of hairy root induction in the medicinal plant Bilhar (Dorema aucheri) using Agrobacterium rhizogenes for the production of secondary metabolites
Fatemeh Jodaki , Seyed Ahmad Sadat Noori * , Seyed Mohammad Mahdi Mortazavian , Mahdi Mahmoudi
Department of Agronomy and Plant Breeding, Abu Raihan Faculty of Agricultural Technology, University of Tehran, Pakdasht, Iran , noori@ut.ac.ir
Abstract:   (1087 Views)
Dorema aucheri, commonly known as Bilhar, is a perennial medicinal plant belonging to the Apiaceae family, known for its diverse and significant medicinal properties. The aim of this study is to investigate the induction of hairy roots in the medicinal plant Bilhar using Agrobacterium rhizogenes to optimize the production of secondary metabolites in in vitro conditions. This experiment was conducted factorially within a completely randomized design with three replications. The factors examined included explant type at three levels (hypocotyl, stem, and leaf) and bacterial strain at two levels (ATCC-15834 and A4). The results indicated that the interaction of the investigated factors significantly affected the traits of hairy root induction percentage, as well as the fresh and dry weights of the hairy roots after two months. The highest percentage of hairy root induction, along with the greatest fresh and dry weight, was achieved with the combination of the ATCC-15834 strain and hypocotyl explants, which was significantly higher than other treatment combinations. The transgenic nature of the hairy roots was confirmed by tracing a part of the rolB gene using PCR, while no corresponding band was amplified for natural (non-transgenic) roots. Overall, the findings of this study suggest that hairy root induction in Bilhar is influenced by both explant type and bacterial strain, and these results are an important prerequisite for experiments related to hairy root culture aimed at producing medicinal metabolites.
 
Keywords: Bilhar, Hairy roots, Agrobacterium rhizogenes, Secondary metabolites
Full-Text [PDF 589 kb]   (167 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2024/11/29 | Accepted: 2025/03/10 | Published: 2025/08/4
References
1. Ajani, Y., & Claβen-Bockhoff, R. (2021). The unique inflorescence structure of Dorema aucheri (Apiaceae): an adaptation to the arid environment. Journal of Arid Environments, 184, 104-194. doi.org/10.1016/j.jaridenv.2020.104194 [DOI:10.1016/j.jaridenv.2020.104194]
2. Ahangarpour, A., Zamaneh, H. T., Jabari, A., Nia, H. M., & Heidari, H. (2014). Antidiabetic and hypolipidemic effects of Dorema aucheri hydroalcoholic leave extract in streptozotocin-nicotinamide induced type 2 diabetes in male rats. Iranian journal of basic medical sciences, 17(10), 808.‏14 doi.org/10.22038/ijbms.2014.1428 [In Persian]
3. Akhavan Roofigar, A., Khazaei, Z., Asadi-Corom, F., & Jalili, A. (2023). Chromosome numbers and karyotype features of eight species of Apiaceae in Iran. The Iranian Journal of Botany, 29(1), 73-77. doi.org/10.22092/ijb.2023.362115.1408 [In Persian]
4. Dorani, E., & Aharizad, S. (2018). Effects of Agrobacterium rhizogenes strains and explants on induction of hair root in Physalis alkekengi. Genetic Engineering and Biosafety Journal, 7(1), 33-40. dorl.net/dor/20.1001.1.25885073.1397.7.1.2.1 [In Persian]
5. Cheng, Y., Wang, X., Cao, L., Ji, J., Liu, T., & Duan, K. (2021). Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean. Plant Methods, 17, 1-12. doi.org/10.1186/s13007-021-00778-7 [DOI:10.1186/s13007-021-00778-7] [PMID] [PMCID]
6. Etebari, M., Sajjadi, S. E., Jafarian-Dehkordi, A., & Nazmakanipour, S. (2016). Genotoxicity evaluation of hydroalcoholic and aqueous extracts of Dorema aucheri by the comet assay. Advanced biomedical research, 27(5), 199.‏ PMID: 28217637. doi.org/10.4103/2277-9175.194830 [DOI:10.4103/2277-9175.190993] [PMID] [PMCID]
7. Georgiev, M. I., Pavlov, A. I., & Bley, T. (2007). Hairy root type plant in vitro systems as sources of bioactive substances. Applied microbiology and biotechnology, 74, 1175-1185. doi.org/10.1007/s00253-007-0856-5 [DOI:10.1007/s00253-007-0856-5] [PMID]
8. Kubica, P., Szopa, A., Kokotkiewicz, A., Miceli, N., Taviano, M. F., Maugeri, A., ... & Ekiert, H. (2020). Production of verbascoside, isoverbascoside and phenolic acids in callus, suspension, and bioreactor cultures of Verbena officinalis and biological properties of biomass extracts. Molecules, 25(23), 5609. doi.org/10.3390/molecules25235609 [DOI:10.3390/molecules25235609] [PMID] [PMCID]
9. Kiselev, K. V., Dubrovina, A. S., Veselova, M. V., Bulgakov, V. P., Fedoreyev, S. A., & Zhuravlev, Y. N. (2007). The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells. Journal of biotechnology, 128(3), 681-692. doi: 10.1016/j.jbiotec.2006.11.008 [DOI:10.1016/j.jbiotec.2006.11.008] [PMID]
10. Soltaninezhad, N., Sadat-Noori, S. A., Izady-Darbandi, A., Amini, F., & Mirjalili, M. H. (2023). Induction and study of hairy root growth pattern in Indian ginseng medicinal plant using Agrobacterium rhizogenes. Genetic Engineering and Biosafety Journal, 12(2), 184-194. dor: 20.1001.1.25885073.1402.12.1.4.8 [In Persian] [DOI:10.61186/gebsj.12.2.184]
11. Mahmoudi, M., Sadat-Noori, S.A., Ebrahimi, M., & Bahmankar, M. (2023). Optimization of induction of hairy roots in Perilla. Genetic Engineering and Biosafety Journal; 12 (1) : 17-27. dor: 20.1001.1.25885073.1402.12.1.4.8
12. Majumdar, S., Garai, S., & Jha, S. (2011). RETRACTED ARTICLE: Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants. Plant Cell Reports, 30(5), 941-954.‏ doi:10.1007/s00299-011-1035-9 [DOI:10.1007/s00299-011-1035-9] [PMID]
13. Mirzaie Delbari, E., Vatandoost, J., Jami Moeini, M., & Kohan-Baghkheirati, E. (2022). Effect of Agrobacterium rhizogenes Strains on Hairy Root Induction in Different Explants of Alfalfa. Applied Biology, 35(3), 127-142. Doi:¬ 10.22051/JAB.2021.34309.1398
14. Moradi, N., Sadat Noori, S. A., Fadavi, A., Mortazavian, S. M. M., & Pakdin Parizi, A. (2021). Analysis efficiency of Iranian Ajowan ecotypes on hairy root production mediated by different Agrobacterium rhizogenesis strains. Iranian Journal of Genetics and Plant Breeding, 10(1), 117-127. doi: 10.30479/IJGPB.2022.17488.1325.
15. Oksman-Caldentey, K. M., & Hiltunen, R. (1996). Transgenic crops for improved pharmaceutical products. Field crops research, 45(1-3), 57-69. doi: 10.1016/0378-4290(95)00059-3 [DOI:10.1016/0378-4290(95)00059-3]
16. Paolis, A. D., Frugis, G., Giannino, D., Iannelli, M. A., Mele, G., Rugini, E., ... & Caretto, S. (2019). Plant cellular and molecular biotechnology: following Mariotti's steps. Plants, 8(1), 18.‏ doi: 10.3390/plants8010018 [DOI:10.3390/plants8010018] [PMID] [PMCID]
17. Potrykus, I. (1990). Gene transfer to plants: assessment and perspectives. Physiologia Plantarum, 79(1), 125-134. doi.org/10.1111/j.1399-3054. 1990.tb 04573.x https://doi.org/10.1034/j.1399-3054.1990.790117.x [DOI:10.1111/j.1399-3054.1990.tb05874.x]
18. Stepanova, A. Y., Malunova, M. V., Gladkov, E. A., Evsyukov, S. V., Tereshonok, D. V., & Solov'eva, A. I. (2022). Collection of hairy roots as a basis for fundamental and applied research. Molecules, 27(22), 8040. doi.org/10.3390/molecules27228040 [DOI:10.3390/molecules27228040] [PMID] [PMCID]
19. Soltaninezhad, N., Sadat-Noori, S. A., Izady-Darbandi, A., Amini, F., & Mirjalili, M. H. (2023). Induction and study of hairy root growth pattern in Indian ginseng medicinal plant using Agrobacterium rhizogenes. Genetic Engineering and Biosafety Journal, 12(2), 184-194. dx.doi.org/10.61186/gebsj.12.2.184 [In Persian] [DOI:10.61186/gebsj.12.2.184]
20. Sujatha, G., Zdravković-Korać, S., Ćalić, D., Flamini, G., & Kumari, B. R. (2013). High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: hairy root production and essential oil analysis. Industrial Crops and Products, 44, 643-652. doi: 10.1016/j.indcrop.2012.09.007 [DOI:10.1016/j.indcrop.2012.09.007]
21. Tiwari, R. K., Trivedi, M., Guang, Z. C., Guo, G. Q., & Zheng, G. C. (2007). Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures. Plant Cell Reports, 26(2), 199-210. doi: 10.1007/s00299-006-0236-0 [DOI:10.1007/s00299-006-0236-0] [PMID]
22. Wahyuni, D. K., Vidianti, F., Purnobasuki, H., Ermayanti, T. M., Prajoga, B., & Utami, E. S. W. (2015). Agrobacterium rhizogenes mediated hairy root induction in Justicia gendarussa Burm. f. Journal of Applied Environmental and Biological Sciences, 5(4), 87-93. doi:10.1186/s13007-021-00778-7 [DOI:10.1186/s13007-021-00778-7] [PMID] [PMCID]
23. Yazdi, F. T., Behbahani, B. A., Vasiee, A., Mortazavi, S. A., & Yazdi, F. T. (2015). An investigation on the effect of alcoholic and aqueous extracts of Dorema aucheri (Bilhar) on some pathogenic bacteria in vitro. Archives of Advances in Biosciences, 6(1), ‏58-64. doi: 10.22037/jps-v6i1.37295¬ [In Persian]
24. Zibaee, E., Amiri, M. S., Boghrati, Z., Farhadi, F., Ramezani, M., Emami, S. A., & Sahebkar, A. (2020). Ethnomedicinal uses, phytochemistry and pharmacology of Dorema species (Apiaceae): A review. Journal of Pharmacopuncture, 23(3), 91-123. doi: 10.3831/KPI.2020.23.3.91 [DOI:10.3831/KPI.2020.23.3.91] [PMID] [PMCID]
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Jodaki F, Sadat Noori S A, Mortazavian S M M, Mahmoudi M. Optimization of hairy root induction in the medicinal plant Bilhar (Dorema aucheri) using Agrobacterium rhizogenes for the production of secondary metabolites. gebsj 2025; 13 (2) :180-189
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Volume 13, Issue 2 (1-2025) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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