Effects of plant growth regulators, genotype and explant on in vitro organogenesis of soybean (Glycine max L.)

Mehrizadeh, Vahid; Dorani, Ebrahim

doi:10.61882/gebsj.13.2.8

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Volume 13, Issue 2 (1-2025)

gebsj 2025, 13(2): 235-245

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Effects of plant growth regulators, genotype and explant on in vitro organogenesis of soybean (Glycine max L.)

Vahid Mehrizadeh

, Ebrahim Dorani ^*

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran , dorani@tabrizu.ac.ir

Abstract: (1095 Views)

Soybean is one of the most important crops and nutrient source of oil and protein in the world. Soybean breeding trough biotechnology is one of the current manners to overcome cultivation and qualitative problems of soybean production. In this study effect of genotype (Williams, Sari and DPX), explant (hypocotyl, cotyledon and first leaf) and plant growth regulators such as BAP (0.5, 1.0, 1.5 and 2.0 mg/L) and IBA (0.0, 0.1 and 0.5 mg/L) on in vitro organogenesis of soybean investigated. This experiment was performed as a factorial in a completely randomized design (CRD) with four replications. Analysis of variance for organogenesis parameters showed that there was significant difference between genotypes, explants and PGR combinations at one percent significance level. Highest organogenesis obtained from cotyledon explants in MS medium supplemented with 1.5 and 2.0 mg/L BAP plus 0.1 mg/L IBA in Williams. The highest soot per explant have been recorded in medium supplemented with 1.5 mg/L BAP plus 0.1 mg/L IBA in Williams using cotyledon explants. Finally, the regenerated shots transferred to 1/2 MS medium supplemented with 1.0 mg/L IBA for root induction and rooted plants adapted in greenhouse. The optimal results obtained in this study can provide the basis for future research, especially gene transfer studies to these soybean genotypes.

Keywords: Soybean, Organogenesis, Genotype, Plant Growth Regulators

Full-Text [PDF 976 kb] (288 Downloads)

Type of Study: Research | Subject: Plant
Received: 2025/02/27 | Accepted: 2025/09/3 | Published: 2025/09/14

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Mehrizadeh V, Dorani E. Effects of plant growth regulators, genotype and explant on in vitro organogenesis of soybean (Glycine max L.). gebsj 2025; 13 (2) :235-245
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