|
Green Synthesis of ZnO and CuO Nanoparticles Using Extract of Moringa and Evaluation of Their Effects on Mo-CBP3 Gene Expression
|
Salehe Ganjali    , Hamide Khajeh , Ayoub Mazarei * , Hossein Kamaladini , Hassan Ahmadi |
| Department of Plant Breeding and Biotechnology University of Zabol , Mazaraie70@gmail.com |
|
|
Abstract: (138 Views) |
The use of plant extracts for the green synthesis of nanoparticles is an environmentally friendly approach that employs natural solvents and, due to increased production efficiency and reduced cost, time, and energy, can serve as a suitable alternative to conventional physical and chemical methods. This study aimed to synthesize zinc oxide (ZnO) and copper oxide (CuO) nanoparticles using Moringa plant extract and to evaluate the effects of different concentrations (0, 100, 150, and 250 ppm) on the relative expression of the Mo-CBP3 gene in Moringa at 48 and 72 hours after treatment. Structural characterization of the nanoparticles using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis showed that the synthesized particles were spherical with sizes of 25–37 nm for ZnO and 32–36 nm for CuO. Analysis of variance revealed that application of ZnO and CuO nanoparticles altered Mo-CBP3 gene expression. The highest relative expression (1.51-fold) was observed 48 hours after treatment with 150 ppm ZnO, showing a 6.67% increase compared to the control. Increasing the ZnO concentration led to a significant decrease in gene expression at 72 hours, while 100 ppm CuO induced the highest relative expression (1.504-fold) at 48 hours, corresponding to a 6.55% increase over the control. In combined treatments, co-application of 150 ppm ZnO and 100 ppm CuO resulted in the highest relative Mo-CBP3 expression at 48 hours, showing a 2.73% increase compared to the control. Overall, these results indicate that ZnO and CuO nanoparticles act as effective elicitors for enhancing the expression of the antifungal Mo-CBP3 gene, with the most pronounced effect observed 48 hours after treatment. |
|
| Keywords: Green synthesis, Medicinal plants, Scanning Electron Microscopy, Nano elicitor, Real Time PCR |
|
|
|
Type of Study: Research |
Subject:
Plant
Received: 2025/04/26 | Accepted: 2025/10/23 | Published: 2025/10/28
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
