[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Archive
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 14, Issue 1 (9-2025) ::
gebsj 2025, 14(1): 60-76 Back to browse issues page
Green Synthesis of ZnO and CuO Nanoparticles Using Extract of Moringa and Evaluation of Their Effects on Mo-CBP3 Gene Expression
Salehe Ganjali , Hamide Khajeh , Ayoub Mazarei * , Hossein Kamaladini , Hassan Ahmadi
Department of Plant Breeding and Biotechnology University of Zabol , Mazaraie70@gmail.com
Abstract:   (729 Views)
The use of plant extracts for the green synthesis of nanoparticles is an environmentally friendly approach that employs natural solvents and, due to increased production efficiency and reduced cost, time, and energy, can serve as a suitable alternative to conventional physical and chemical methods. This study aimed to synthesize zinc oxide (ZnO) and copper oxide (CuO) nanoparticles using Moringa plant extract and to evaluate the effects of different concentrations (0, 100, 150, and 250 ppm) on the relative expression of the Mo-CBP3 gene in Moringa at 48 and 72 hours after treatment. Structural characterization of the nanoparticles using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis showed that the synthesized particles were spherical with sizes of 25–37 nm for ZnO and 32–36 nm for CuO. Analysis of variance revealed that application of ZnO and CuO nanoparticles altered Mo-CBP3 gene expression. The highest relative expression (1.51-fold) was observed 48 hours after treatment with 150 ppm ZnO, showing a 6.67% increase compared to the control. Increasing the ZnO concentration led to a significant decrease in gene expression at 72 hours, while 100 ppm CuO induced the highest relative expression (1.504-fold) at 48 hours, corresponding to a 6.55% increase over the control. In combined treatments, co-application of 150 ppm ZnO and 100 ppm CuO resulted in the highest relative Mo-CBP3 expression at 48 hours, showing a 2.73% increase compared to the control. Overall, these results indicate that ZnO and CuO nanoparticles act as effective elicitors for enhancing the expression of the antifungal Mo-CBP3 gene, with the most pronounced effect observed 48 hours after treatment.
Keywords: Green synthesis, Medicinal plants, Scanning Electron Microscopy, Nano elicitor, Real Time PCR
Full-Text [PDF 1066 kb]   (155 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2025/04/26 | Accepted: 2025/10/23 | Published: 2025/10/28
References
1. Abdallah, R., Mostafa, N. Y., Kirrella, G. A., Gaballah, I., Imre, K., Morar, A., ... & Elshebrawy, H. A. (2023). Antimicrobial effect of Moringa oleifera leaves extract on foodborne pathogens in ground beef. Foods, 12(4), 766. https://doi.org/10.3390/foods12040766 [DOI:10.3390/foods12040766.] [PMID] [PMCID]
2. Alharbi, N. S., Alsubhi, N. S., & Felimban, A. I. (2022). Green synthesis of silver nanoparticles using medicinal plants: Characterization and application. Journal of Radiation Research and Applied Sciences, 15(3), 109-124. [DOI:10.1016/j.jrras.2022.06.012]
3. Assad, N., Laila, M. B., Hassan, M. N. U., Rehman, M. F. U., Ali, L., Mustaqeem, M., ... & Malik, T. (2025). Eco-friendly synthesis of gold nanoparticles using Equisetum diffusum D. Don. with broad-spectrum antibacterial, anticancer, antidiabetic, and antioxidant potentials. Scientific Reports, 15(1), 19246. [DOI:10.1038/s41598-025-02450-9] [PMID] [PMCID]
4. Babaei, Z., Solouki, M., & Fazeli-Nasab, B. (2019). Investigating The Effect of Biological and non-Biological Elicitor on Expression of Hyp-1 Gene in Hypericum perforatum. Modern Genetics, 13(4), 543-549‎. DOR: 20.1001.1.20084439.1397.13.4.9.2. In Persian
5. Babajani, A., Iranbakhsh, A., Oraghi Ardebili, Z., & Eslami, B. (2019). Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles. Environmental Science and Pollution Research, 26(24), 24430-24444. [DOI:10.1007/s11356-019-05676-z] [PMID]
6. Banerjee, K., Pramanik, P., Maity, A., Joshi, D. C., Wani, S. H., & Krishnan, P. (2019). Methods of using nanomaterials to plant systems and their delivery to plants (mode of entry, uptake, translocation, accumulation, biotransformation and barriers). In Advances in phytonanotechnology (pp. 123-152). Academic Press. [DOI:10.1016/B978-0-12-815322-2.00005-5] [PMID] [PMCID]
7. Batista, A. B., Oliveira, J. T., Gifoni, J. M., Pereira, M. L., Almeida, M. G., Gomes, V. M., ... & Vasconcelos, I. M. (2014). New insights into the structure and mode of action of Mo-CBP3, an antifungal chitin-binding protein of Moringa oleifera seeds. PloS one, 9(10), e111427. [DOI:10.1371/journal.pone.0111427] [PMID] [PMCID]
8. Behjati, M. M., Nemati, A., & Ardalan, P. (2020). Evaluation of the ZnO nanoparticles biosynthesized by Amaranthus Ruentus on the expression of apoptotic genes (Bax and Bcl-2) in breast cancer cells (MDAMB-231). DOR: ‎ 20.1001.1.22285105.2019.9.4.11.0. In Persian
9. Bindhu MR, Umadevi M, Esmail GA, Al-Dhabi NA, Arasu MV. Green synthesis and characterization of silver nanoparticles from Moringa oleifera flower and assessment of antimicrobial and sensing properties. J Photochem Photobiol B Biol. 2020;205:111836. [DOI:10.1016/j.jphotobiol.2020.111836] [PMID]
10. Chomoucka, J., Drbohlavova, J., Huska, D., Adam, V., Kizek, R., & Hubalek, J. (2010). Magnetic nanoparticles and targeted drug delivering. Pharmacological research, 62(2), 144-149. [DOI:10.1016/j.phrs.2010.01.014] [PMID]
11. Da Costa, M. V. J., & Sharma, P. K. (2016). Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa. Photosynthetica, 54, 110-119. [DOI:10.1007/s11099-015-0167-5]
12. Das PE, Majdalawieh AF, Abu-Yousef IA, Narasimhan S, Poltronieri P. Use of a hydroalcoholic extract of moringa oleifera leaves for the green synthesis of bismuth nanoparticles and evaluation of their anti-microbial and antioxidant activities. Materials (Basel). 2020;13:876. [DOI:10.3390/ma13040876] [PMID] [PMCID]
14. Dejene, B. K., & Geletaw, T. M. (2023). A review of plant-mediated synthesis of zinc oxide nanoparticles for self-cleaning textiles. Research Journal of Textile and Apparel. [DOI:10.1108/RJTA-12-2022-0154]
15. Deresa, E. M., & Diriba, T. F. (2023). Phytochemicals as alternative fungicides for controlling plant diseases: A comprehensive review of their efficacy, commercial representatives, advantages, challenges for adoption, and possible solutions. Heliyon, 9(3). [DOI:10.1016/j.heliyon.2023.e13810] [PMID] [PMCID]
16. Espenti CS, Rama Krishna AG, Rami Reddy YV. Green biosynthesis of ZnO nanomaterials and their anti-bacterial activity by using Moringa Oleifera root aqueous extract. SN Appl Sci. 2020;2:1-11. [DOI:10.1016/j.molliq.2024.126483]
17. Falowo, A.B.; Muchenje, V.; Hugo, A.; Aiyegoro, O.A.; Fayemi, P.O. Antioxidant activities of Moringa oleifera L. and Bidens pilosa L. leaf extracts and their effects on oxidative stability of ground raw beef during refrigeration storage. CyTA J. Food 2017, 15, 249-256. [DOI:10.1080/19476337.2016.1243587]
18. Farahani, S., Bandani, A., & Eslami, S. (2018). Comparison of susceptibility of two Iranian populations of Tetranychus urticae Koch (Acari: Tetranychidae) to spirodiclofen. [DOI:10.22073/pja.v7i3]
19. Freire reire, J. E. et al., Mo-CBP3, an antifungal chitin-binding protein from Moringa oleifera seeds, is a member of the 2S albumin family, PLoS ONE, 2015. [DOI:10.1371/journal.pone.0119871] [PMID] [PMCID]
20. Freire, J. E., Vasconcelos, I. M., Moreno, F. B., Batista, A. B., Lobo, M. D., Pereira, M. L., ... & Grangeiro, T. B. (2015). Mo-CBP3, an antifungal chitin-binding protein from Moringa oleifera seeds, is a member of the 2S albumin family. PloS one, 10(3), e0119871. [DOI:10.1371/journal.pone.0119871] [PMID] [PMCID]
21. Ghahremani, F., & Izanloo, C. (2020). Green synthesis of Copper Oxide Nanopartcles Using Extract of Hypericum Perforatum and Marrubium Vulgare and Evaluation of Antioxidant Properties of Herbal Extracts and Antibacterial Feature of Green-Synthesized Nanostructures. , 12(44), 239-249. DOR: 20.1001.1.20086156.1399.12.44.3.6. In Persian
22. Ghebremichael, K. A., Gunaratna, K. R., Henriksson, H., Brumer, H., & Dalhammar, G. (2005). A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water research, 39(11), 2338-2344. [DOI:10.1016/j.watres.2005.04.012] [PMID]
23. Gifoni, J. M., Oliveira, J. T., Oliveira, H. D., Batista, A. B., Pereira, M. L., Gomes, A. S., ... & Vasconcelos, I. M. (2012). A novel chitin‐binding protein from Moringa oleifera seed with potential for plant disease control. Peptide Science, 98(4), 406-415. [DOI:10.1002/bip.22068] [PMID]
24. Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food science and human wellness, 5(2), 49-56. [DOI:10.1016/j.fshw.2016.04.001] [PMCID]
25. Hernández-Ceja, A., Loeza-Lara, P. D., Espinosa-García, F. J., García-Rodríguez, Y. M., Medina-Medrano, J. R., Gutiérrez-Hernández, G. F., & Ceja-Torres, L. F. (2021). In vitro antifungal activity of plant extracts on pathogenic fungi of blueberry (Vaccinium sp.). Plants, 10(5), 852. [DOI:10.3390/plants10050852] [PMID] [PMCID]
27. Hernández-Hernández, H., Juárez-Maldonado, A., Benavides-Mendoza, A., Ortega-Ortiz, H., Cadenas-Pliego, G., Sánchez-Aspeytia, D., & González-Morales, S. (2018). Chitosan-PVA and copper nanoparticles improve growth and overexpress the SOD and JA genes in tomato plants under salt stress. Agronomy, 8(9), 175. [DOI:10.3390/agronomy8090175]
28. Jaiswal, D., Rai, P. K., Mehta, S., Chatterji, S., Shukla, S., Rai, D. K., ... & Watal, G. (2013). Role of Moringa oleifera in regulation of diabetes-induced oxidative stress. Asian Pacific journal of tropical medicine, 6(6), 426-432. [DOI:10.1016/S1995-7645(13)60068-1] [PMID]
29. Javed, R., Khan, B., Sharafat, U., Bilal, M., Galagedara, L., Abbey, L., & Cheema, M. (2024). Dynamic interplay of metal and metal oxide nanoparticles with plants: Influencing factors, action mechanisms, and assessment of stimulatory and inhibitory effects. Ecotoxicology and Environmental Safety, 271, 115992. [DOI:10.1016/j.ecoenv.2024.115992] [PMID]
30. Jośko, I., Kusiak, M., Xing, B., & Oleszczuk, P. (2021). Combined effect of nano-CuO and nano-ZnO in plant-related system: From bioavailability in soil to transcriptional regulation of metal homeostasis in barley. Journal of hazardous materials, 416, 126230. [DOI:10.1016/j.ecoenv.2024.115992] [PMID]
31. Kaur, H., Sharma, A., Anand, K., Panday, A., Tagotra, S., Kakran, S., ... & Singh, G. (2025). Green synthesis of ZnO nanoparticles using E. cardamomum and zinc nitrate precursor: a dual-functional material for water purification and antibacterial applications. RSC advances, 15(21), 16742-16765. [DOI:10.1039/D5RA01469G] [PMID] [PMCID]
32. Ke, M., Zhu, Y., Zhang, M., Gumai, H., Zhang, Z., Xu, J., & Qian, H. (2017). Physiological and molecular response of Arabidopsis thaliana to CuO nanoparticle (nCuO) exposure. Bulletin of environmental contamination and toxicology, 99(6), 713-718. [DOI:10.1007/s00128-017-2205-4] [PMID]
33. Khodayari, M., Omidi, M., Shah Nejat Bushehri, A., Yazdani, D., Naqvi, M. R., & Kadkhoda, Z. (2014). Effect biological elicitor and nano elicitor on increasing the production of alkaloids in opium poppy (Papaver somniferum). Iranian Horticultural Science, 45, 287-295. [DOI:10.22059/ijhs.2014.52877. In Persian]
34. Kim, S. H., Bae, S., Sung, Y. W., & Hwang, Y. S. (2024). Effects of particle size on toxicity, bioaccumulation, and translocation of zinc oxide nanoparticles to bok choy (Brassica chinensis L.) in garden soil. Ecotoxicology and Environmental Safety, 280, 116519. [DOI:10.1016/j.ecoenv.2024.116519] [PMID]
35. Kister, T., Monego, D., Mulvaney, P., Widmer-Cooper, A., & Kraus, T. (2018). Colloidal stability of apolar nanoparticles: The role of particle size and ligand shell structure. ACS nano, 12(6), 5969-5977. [DOI:10.1021/acsnano.8b02202] [PMID]
36. Lala, S. (2021). Nanoparticles as elicitors and harvesters of economically important secondary metabolites in higher plants: A review. IET nanobiotechnology, 15(1), 28-57. [DOI:10.1049/nbt2.12005] [PMID] [PMCID]
37. Langner, T., & Göhre, V. (2016). Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions. Current genetics, 62(2), 243-254. [DOI:10.1007/s00294-015-0530-x] [PMID]
38. Leopold, L. F., Coman, C., Clapa, D., Oprea, I., Toma, A., Iancu, Ș. D., ... & Coman, V. (2022). The effect of 100-200 nm ZnO and TiO2 nanoparticles on the in vitro-grown soybean plants. Colloids and Surfaces B: Biointerfaces, 216, 112536. [DOI:10.1016/j.colsurfb.2022.112536] [PMID]
39. Lv, Z., Jiang, R., Chen, J., & Chen, W. (2020). Nanoparticle‐mediated gene transformation strategies for plant genetic engineering. The Plant Journal, 104(4), 880-891. [DOI:10.1111/tpj.14973] [PMID]
40. Madariaga-Mazón, A., Hernández-Alvarado, R. B., Noriega-Colima, K. O., Osnaya-Hernández, A., & Martinez-Mayorga, K. (2019). Toxicity of secondary metabolites. Physical Sciences Reviews, 4(12). [DOI:10.1515/psr-2018-0116]
41. Mahdi, H. J., Khan, N. A. K., Asmawi, M. Z. B., & Mahmud, R. (2018). In vivo anti-arthritic and anti-nociceptive effects of ethanol extract of Moringa oleifera leaves on complete Freund's adjuvant (CFA)-induced arthritis in rats. Integrative medicine research, 7(1), 85-94. [DOI:10.1016/j.imr.2017.11.002] [PMID] [PMCID]
42. Meela, M. M., Mdee, L. K., Masoko, P., & Eloff, J. N. (2019). Acetone leaf extracts of seven invasive weeds have promising activity against eight important plant fungal pathogens. South African journal of botany, 121, 442-446. [DOI:10.1016/j.sajb.2018.12.007]
43. Michen, B., Geers, C., Vanhecke, D., Endes, C., Rothen-Rutishauser, B., Balog, S., & Petri-Fink, A. (2015). voiding drying-artifacts in transmission electron microscopy: Characterizing the size and colloidal state of nanoparticles. Scientific reports, 5(1), 9793. [DOI:10.1038/srep09793] [PMID] [PMCID]
44. Minaiyan, M., Asghari, G., Taheri, D., Saeidi, M., & Nasr-Esfahani, S. (2014). Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats. Avicenna journal of phytomedicine, 4(2), 127. [DOI:10.22038/ajp.2014.1072]
45. Moghadami, F., Hajmoradi, F., & Kalantari, M. (2023). Investigating the effect of silver nanoparticles on the activity of glycerol dehydrogenase by response surface methodology. Genetic Engineering and Biosafety Journal, 12(1), 59-67. DOR: 20.1001.1.25885073.1402.12.1.7.1. In Persian
46. Moore, T. L., Rodriguez-Lorenzo, L., Hirsch, V., Balog, S., Urban, D., Jud, C., ... & Petri-Fink, A. (2015). Nanoparticle colloidal stability in cell culture media and impact on cellular interactions. Chemical Society Reviews, 44(17), 6287-6305. . https://doi.org/ 10.1039/C4CS00487F [DOI:10.1039/C4CS00487F] [PMID]
47. Mosa, K. A., El-Naggar, M., Ramamoorthy, K., Alawadhi, H., Elnaggar, A., Wartanian, S., ... & Hani, H. (2018). Copper nanoparticles induced genotoxicty, oxidative stress, and changes in superoxide dismutase (SOD) gene expression in cucumber (Cucumis sativus) plants. Frontiers in plant science, 9, 872. [DOI:10.3389/fpls.2018.00872] [PMID] [PMCID]
48. Naghavi,F. , Khoshroo,S. M. R. , Kazemipour,M. and Mahmoudi Zarandi,M. (2024). Effect of green copper nanoparticles synthesized with Aloe vera aqueous extract on germination parameters of pinto beans under salinity stress. Journal of Plant Research (Iranian Journal of Biology), 37(1), 16-29. https://doi.org 10.22034/jpr.2024.2249. . In Persian
49. Naiel, B., Fawzy, M., Halmy, M. W. A., & Mahmoud, A. E. D. (2022). Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials. Scientific Reports, 12(1), 20370. [DOI:10.1038/s41598-022-24805-2] [PMID] [PMCID]
50. Othman, M., Saada, H., & Matsuda, Y. (2020). Antifungal activity of some plant extracts and essential oils against fungi‐infested organic archaeological artefacts. Archaeometry, 62(1), 187-199. [DOI:10.1111/arcm.12500]
51. Paikra, B. K., & Gidwani, B. (2017). Phytochemistry and pharmacology of Moringa oleifera Lam. Journal of pharmacopuncture, 20(3), 194. [DOI:10.3831/KPI.2017.20.022] [PMID] [PMCID]
52. Perumalsamy, H., Balusamy, S. R., Sukweenadhi, J., Nag, S., MubarakAli, D., El-Agamy Farh, M., ... & Rahimi, S. (2024). A comprehensive review on Moringa oleifera nanoparticles: importance of polyphenols in nanoparticle synthesis, nanoparticle efficacy and their applications. Journal of nanobiotechnology, 22(1), 71. [DOI:10.1186/s12951-024-02332-8] [PMID] [PMCID]
53. Raafat, K., & Hdaib, F. (2017). Neuroprotective effects of Moringa oleifera: Bio-guided GC-MS identification of active compounds in diabetic neuropathic pain model. Chinese journal of integrative medicine, 1-10. [DOI:10.1007/s11655-017-2758-4] [PMID]
54. Radwan, A. M., Aboelfetoh, E. F., Kimura, T., Mohamed, T. M., & El-Keiy, M. M. (2021). Fenugreek-mediated synthesis of zinc oxide nanoparticles and evaluation of its in vitro and in vivo antitumor potency. Biomedical Research and Therapy, 8(8), 4483-4496. [DOI:10.15419/bmrat.v8i8.687]
55. Rahimi Sherbaf Moghadas, M., Naghavi, M., Sabokdast, M., Motemadi, E., & Nasiri, J. (2021). The effect of nano elicitors on the expression of the genes involved in alkaloids biosynthetic pathway in Papaver orientale L. suspension culture. Iranian Journal of Field Crop Science, 52(1), 133-141. [DOI:10.22059/ijfcs.2020.256665.654466. In Persian]
56. Rezaee, M., Hosseini, R., & Asghari, B. (2016). Evaluating the effect of zinc and cobalt nanoparticles on expression of STR, DAT and D4H genes in periwinkle (Cataranthus roseus) suspenssion culture. Cell and Tissue Journal, 7(4), 355-364. [DOI:10.52547/JCT.7.4.355. In Persian]
57. Rizwan, M., Ali, S., Qayyum, M. F., Ok, Y. S., Adrees, M., Ibrahim, M., ... & Abbas, F. (2017). Effect of metal and metal oxide nanoparticles on growth and physiology of globally important food crops: A critical review. Journal of hazardous materials, 322, 2-16. [DOI:10.1016/j.jhazmat.2016.05.061] [PMID]
58. Singh, H., Desimone, M. F., Pandya, S., Jasani, S., George, N., Adnan, M., ... & Alderhami, S. A. (2023). Revisiting the green synthesis of nanoparticles: uncovering influences of plant extracts as reducing agents for enhanced synthesis efficiency and its biomedical applications. International journal of nanomedicine, 4727-4750. [DOI:10.2147/IJN.S419369] [PMID] [PMCID]
59. Teixeira, E. M. B., Carvalho, M. R. B., Neves, V. A., Silva, M. A., & Arantes-Pereira, L. (2014). Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. leaves. Food chemistry, 147, 51-54. [DOI:10.1016/j.foodchem.2013.09.135] [PMID]
60. Tripathi, S., Mahra, S., Tiwari, K., Rana, S., Tripathi, D. K., Sharma, S., & Sahi, S. (2023). Recent advances and perspectives of nanomaterials in agricultural management and associated environmental risk: a review. Nanomaterials, 13(10), 1604. [DOI:10.3390/nano13101604] [PMID] [PMCID]
61. Trontin, J. F., Klimaszewska, K., Morel, A., Hargreaves, C., & Lelu-Walter, M. A. (2016). Molecular aspects of conifer zygotic and somatic embryo development: a review of genome-wide approaches and recent insights. In vitro embryogenesis in higher plants, 167-207. [DOI:10.1007/978-1-4939-3061-6_8] [PMID] [PMCID]
62. Vafaie Moghadam, A., Iranbakhsh, A., Saadatmand, S., Ebadi, M., & Oraghi Ardebili, Z. (2022). New insights into the transcriptional, epigenetic, and physiological responses to zinc oxide nanoparticles in Datura stramonium; potential species for phytoremediation. Journal of Plant Growth Regulation, 41(1), 271-281. [DOI:10.1007/s00344-021-10305-6]
63. Vahedi, H. , Fahmideh, L., Fakheri, BA ., & Fazeli-Nasab. B., (2023). Effect of Chitosan and Silver nanoparticles on expression of Betaamyrin synthase gene in Ajowan. Modern Genetics, 18(2)235-244. URL: http://mg.genetics.ir/article-1-1616-fa.html. In Persian
64. Wang, S., Liu, H., Zhang, Y., & Xin, H. (2015). The effect of CuO NPs on reactive oxygen species and cell cycle gene expression in roots of rice. Environmental Toxicology and Chemistry, 34(3), 554-561. [DOI:10.1002/etc.2826] [PMID]
65. Yao, Q., Wu, C. F., Luo, P., Xiang, X. C., Liu, J. J., Mou, L., & Bao, J. K. (2010). A new chitin-binding lectin from rhizome of Setcreasea purpurea with antifungal, antiviral and apoptosis-inducing activities. Process Biochemistry, 45(9), 1477-1485. [DOI:10.1016/j.procbio.2010.05.026] [PMID] [PMCID]
66. Zhang, H., Demirer, G. S., Zhang, H., Ye, T., Goh, N. S., Aditham, A. J., ... & Landry, M. P. (2019). DNA nanostructures coordinate gene silencing in mature plants. Proceedings of the National Academy of Sciences, 116(15), 7543-7548. [DOI:10.1073/pnas.1818290116] [PMID] [PMCID]
67. Zong, X., Wu, D., Zhang, J., Tong, X., Yin, Y., Sun, Y., & Guo, H. (2022). Size-dependent biological effect of copper oxide nanoparticles exposure on cucumber (Cucumis sativus). Environmental Science and Pollution Research, 29(46), 69517-69526. [DOI:10.1007/s11356-022-20662-8] [PMID]
Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ganjali S, Khajeh H, Mazarei A, Kamaladini H, Ahmadi H. Green Synthesis of ZnO and CuO Nanoparticles Using Extract of Moringa and Evaluation of Their Effects on Mo-CBP3 Gene Expression. gebsj 2025; 14 (1) :60-76
URL: http://gebsj.ir/article-1-522-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 1 (9-2025) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
Persian site map - English site map - Created in 0.09 seconds with 38 queries by YEKTAWEB 4758