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:: Volume 4, Issue 2 (10-2015) ::
2015, 4(2): 103-111 Back to browse issues page
Assessment of hairy roots induction of the medicinal plant Alecost (Tanacetum balsamita L.) using Agrobacterium rhizogenes
Asad Maroufi *, Mohammad Majdi
University of Kurdistan, Sanandaj, Iran , asad.maroufi@gmail.com
Abstract:   (4308 Views)

alecost (Tanacetum balsamita L.), belongs to the Asteraseae family, is a pharmacologically important species rich in important secondary metabolites including flavones, sesquiterpene lactones, phenylpropane compounds and derivatives, tannins and essential oils. Alecost has been used both fresh or dried as flavouring or food additive. Additionally, it has medicinal properties and is applied in aromatic products. In addition to traditional farming, in vitro hairy root culture has been found to be suitable for the production of secondary metabolites. Therefore, in order to establish a protocol for hairy root culture of alecost, root induction by co-cultivation with Agrobacterium rhizogenes (strain A4) was assessed in this study. Different explants (cotyledon, young leaf, stem and root) showed different responses to hairy root induction by Agrobacterium rhizogenes. Moreover, the frequencies of hairy root induction for different types of explants were considerably different. The induced roots were shown to be transformed by PCR using primers specific for rolB. This is the first report of hairy root induction in alecost and the results may be useful in genetic manipulation of Tanacetum balsamita and use of hairy root culture to produce high-value secondary metabolites.

Keywords: Agrobacterium rhizogenes, Hairy root, Medicinal plant, Secondary metabolites, Tanacetum balsamita
Full-Text [PDF 250 kb]   (5003 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2015/06/4 | Accepted: 2016/09/7 | Published: 2017/01/25
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Maroufi A, Majdi M. Assessment of hairy roots induction of the medicinal plant Alecost (Tanacetum balsamita L.) using Agrobacterium rhizogenes. Genetic Engineering and Biosafety Journal. 2015; 4 (2) :103-111
URL: http://gebsj.ir/article-1-98-en.html

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Volume 4, Issue 2 (10-2015) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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