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Production of ectoine in plants using genetic engineering to reduce the negative effects of abiotic stresses
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Maryam Sadeghi   , Akram Sadeghi * |
| Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran , aksadeghi@abrii.ac.ir |
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Abstract: (5033 Views) |
| Fluctuations in environmental water potential caused by drought and salinity are the most important abiotic stress factors in many natural habitats of organisms. Compatible solutes stabilize biomolecules and whole cells and play as stress-protective agents. Ectoine and its derivative 5-hydroxyectoine as compatible solutes are synthesized widely by the members of the bacteria, a few archaea, and eukarya in response to high salinity and osmolarity. Previously reported that the genetically engineered ectoine synthesis using ectA, ectB or ectC has resulted in increased hyperosmotic tolerance of model plants. In this review, the importance of current and potential applications of ectoines as protecting agents for macromolecules, cells, and tissues, are discussed. Besides, the leading theory for the protection mechanism of ectoines including the exclusion of ectoines molecules from the protein surface, slows the diffusion of water molecules and preserve the native state of the protein were explained. The experimental data of the past decades concerning alleviating drought and salinity stresses by ectoines and the prospects for their use in different fields of modern biotechnology, including genetic engineering techniques, have been summarized in this review article. |
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| Keywords: ectoines, compatible solutes, osmotic stress, genetic engineering |
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Full-Text [PDF 902 kb]
(1006 Downloads)
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Type of Study: Review |
Subject:
Plant
Received: 2019/12/22 | Accepted: 2020/02/10 | Published: 2020/02/10
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