Regeneration Optimization of Cotton commercial cultivars using meristem apex culture

Hamid, Rasmieh; Ghorbanzadeh, Zahra; Marashi, Hassan; Malekzadeh Shafaroudi, Saeid

doi:10.61186/gebsj.12.2.195

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Volume 12, Issue 2 (11-2023)

gebsj 2023, 12(2): 195-202

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Regeneration Optimization of Cotton commercial cultivars using meristem apex culture

Rasmieh Hamid ^*

, Zahra Ghorbanzadeh

, Hassan Marashi

, Saeid Malekzadeh Shafaroudi

Department of Plant Breeding, Cotton Research Institutes of Iran (CRII), Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran , rasihamid@gmail.com

Abstract: (1550 Views)

Cotton (Gossypium hirsutum. L) is an important economic crop worldwide, valued for its rich fibres and its role as a major source of edible oil. However, the resistance of cotton to conventional regeneration protocols is a challenge. Therefore, the development of efficient plant regeneration protocols for recalcitrant species such as cotton, especially from meristem apex explants, is crucial for the advancement of functional genomics and selective breeding using transformation technologies. In this study, we used seeds of Sahil, Varamin, Khurshid, Golestan, Latif, Kashmer and Armaghan varieties. The meristems isolated from these cultivars were cultured on a medium containing MS salts, B5 vitamins and hormones to induce organogenesis. The shoots were then transferred to a branching medium. Optimal branching was observed with a medium enriched with 2 mg/l BAP and 2 mg/l Kin, with no significant differences between varieties and a maximum branching level of 92. In addition, the shoots were transferred to five different rooting media. Statistical analysis showed that a medium containing MS salts, B5 vitamins and 1 mg/l IBA gave the highest rooting rate of 95%. Our study optimised a fast, productive and genotype-independent method for organogenesis and branching from the shoot tip in cotton. The improved rooting rate and the provision of a genotype-independent rooting method are further results of this research that promise significant advances in cotton regeneration protocols.

Keywords: Shoot apex, Cotton, Direct regeneration, BAP, Rooting

Full-Text [PDF 754 kb] (189 Downloads)

Type of Study: Research | Subject: Plant
Received: 2023/05/26 | Accepted: 2023/08/29 | Published: 2024/03/14

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Hamid R, Ghorbanzadeh Z, Marashi H, Malekzadeh Shafaroudi S. Regeneration Optimization of Cotton commercial cultivars using meristem apex culture. gebsj 2023; 12 (2) :195-202
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