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:: Volume 13, Issue 1 (5-2024) ::
gebsj 2024, 13(1): 22-30 Back to browse issues page
Transformation of tomato with Vacuolar Pyrophosphatase gene (AVP1) to enhance salt tolerance
Ebrahim Dorani * , Mahmoud Toorchi
Department of Plant Biotechnology and plant Breeding, Faculty of Agriculture, University of Tabriz, Iran , uliaie@yahoo.com
Abstract:   (407 Views)
Genetic engineering becomes a hopeful tool to develop stress tolerance transgenic plants including salt tolerance transgenic plants. The subject of this study was the transformation of tomato with a vascular antiporter gene using Agrobacterium. The pBZP plasmid harboring AVP1 gene was transferred to E.coli for amplification and then transferred to Agrobacterium for tomato transformation. Cotyledon and hypocotyl explants inoculated with recombinant Agrobacterium transferred to MS medium supplemented with three concentrations of BAP for plant regeneration. Analysis of variance showed there was no interaction between BAP and explant type in regeneration capacity but both explant and BAP concentration in medium had significant effect on transformation efficiency. Mean compression using Duncan multiple rang test at 5 percent level showed that highest regeneration of transgenic plants achieved in MS medium supplemented with 2 mg/L BAP. Regeneration efficiency from Cotyledon explant was more than cotyledonary explants. Genomic DNA of putative transgenic plants extracted and analyzed by PCR for presence of transgene using AVP1 gene specific primers. The results showed 440 bp of AVP1 amplicon in transgenic plants. The transgenic plants transferred to greenhouse for next investigations.  
Keywords: Salt tolerance, cotyledon, vacuole, membrane pomp
Full-Text [PDF 650 kb]   (94 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2023/11/4 | Accepted: 2024/04/29 | Published: 2024/09/17
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Dorani E, Toorchi M. Transformation of tomato with Vacuolar Pyrophosphatase gene (AVP1) to enhance salt tolerance. gebsj 2024; 13 (1) :22-30
URL: http://gebsj.ir/article-1-475-en.html


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Volume 13, Issue 1 (5-2024) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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